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Literature summary for 1.10.3.1 extracted from
- Hemocyanin-derived phenoloxidase activity with broad temperature stability extending into the cold environment in hemocytes of the hair crab Erimacrus isenbeckii (2011), Comp. Biochem. Physiol. B, 159, 103-108.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| lipopolysaccharide | phenoloxidase activity is highest at 0.2 mg/ml | Erimacrus isenbeckii |  |
| lipoteichoic acid | phenoloxidase activity is highest at 0.2 mg/ml | Erimacrus isenbeckii | |
| additional information | beta-1,3-glucan has almost no effect on the phenoloxidase activity | Erimacrus isenbeckii | |
| SDS | when 0.04 mM SDS is added, the phenoloxidase activity starts to increase and peaks at 5 mM SDS (125fold increase) | Erimacrus isenbeckii | |
| Trypsin | when 0.001 mM trypsin is added, the phenoloxidase activity starts to increase and peaks at 0.04 mM trypsin (40fold increase) | Erimacrus isenbeckii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-Phenyl-2-thiourea | 94.4% inhibition at 0.0293 mM | Erimacrus isenbeckii | |
| EDTA | 88.8% inhibition at 3.75 mM | Erimacrus isenbeckii | |
| N,N-diethyldithiocarbamate | 94.4% inhibition at 0.0293 mM | Erimacrus isenbeckii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.96 | - |
L-Dopa | at 22°C, pH not specified in the publication | Erimacrus isenbeckii | |
| 1.15 | - |
catechol | at 22°C, pH not specified in the publication | Erimacrus isenbeckii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | copper-containing enzyme | Erimacrus isenbeckii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
6 * 75000, SDS-PAGE | Erimacrus isenbeckii |
| 448000 | - |
gel filtration | Erimacrus isenbeckii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Erimacrus isenbeckii | - |
hair crab | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography and Sephadex G-75 gel filtration | Erimacrus isenbeckii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hemocyte | - |
Erimacrus isenbeckii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 catechol + O2 | - |
Erimacrus isenbeckii | 2 1,2-benzoquinone + 2 H2O | - |
? | |
| L-dopa + O2 | - |
Erimacrus isenbeckii | dopachrome + H2O | - |
? | |
| additional information | L-tyrosine and hydroquinone are ineffective as substrates | Erimacrus isenbeckii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 75000, SDS-PAGE | Erimacrus isenbeckii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phenoloxidase | - |
Erimacrus isenbeckii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Erimacrus isenbeckii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 80 | the phenoloxidase activity stays almost the same or decreases slightly up to 60°C, and decreases rapidly at 80°C. The average phenoloxidase activity increases at 4°C, 25°C, 40°C, and 60°C in a time-dependent manner, but no increase is observed at 80°C | Erimacrus isenbeckii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | cold-adapted hemocyanin-derived phenoloxidase activity is important to the survival of Erimacrus isenbeckii at low temperature (4°C) | Erimacrus isenbeckii |
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