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Literature summary for 1.10.3.1 extracted from

  • Gasparetti, C.; Faccio, G.; Arvas, M.; Buchert, J.; Saloheimo, M.; Kruus, K.
    Discovery of a new tyrosinase-like enzyme family lacking a C-terminally processed domain: production and characterization of an Aspergillus oryzae catechol oxidase (2009), Appl. Microbiol. Biotechnol., 86, 213-226.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
AoCO4 is expressed in Trichoderma reesei under the strong cbh1 promoter, resulting in high yields of extracellular enzyme, corresponding to 1.5 g/l production of the enzyme Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39350
-
MALDI-TOF Aspergillus oryzae
40480
-
MALDI-TOF Aspergillus oryzae
53000
-
SDS-PAGE Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the N-terminal analysis of the protein reveals N-terminal processing taking place in the Kex2/furin-type protease cleavage site and removing the first 51 amino acids from the putative N-terminus Aspergillus oryzae

Purification (Commentary)

Purification (Comment) Organism
AoCO4 is purified with a two-step purification procedure, consisting of cation and anion exchange chromatography Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
Aspergillus oryzae 2 1,2-benzoquinone + 2 H2O
-
?
4-tert-butylcatechol + O2
-
Aspergillus oryzae ?
-
?
aminophenol + O2
-
Aspergillus oryzae ?
-
?
aniline + O2 enzyme shows also activity after 24 h incubation Aspergillus oryzae ?
-
?
caffeic acid + O2
-
Aspergillus oryzae ?
-
?
guaiacol + O2
-
Aspergillus oryzae ?
-
?
p-cresol + O2 enzyme shows also activity after 24 h incubation Aspergillus oryzae ?
-
?
phenol + O2 enzyme shows also activity after 24 h incubation Aspergillus oryzae ?
-
?
tyrosol + O2 enzyme shows also activity after 24 h incubation Aspergillus oryzae ?
-
?

Synonyms

Synonyms Comment Organism
AoCO4
-
Aspergillus oryzae
catechol oxidase
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-live 20 h at 50°C Aspergillus oryzae
60
-
enzyme shows thermostability up to 60°C, half-live 2 h at 60°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
Aspergillus oryzae
7
-
assay at Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
5 7
-
Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
cosiderable activity loss below pH 5.0 Aspergillus oryzae