Cloned (Comment) | Organism |
---|---|
AoCO4 is expressed in Trichoderma reesei under the strong cbh1 promoter, resulting in high yields of extracellular enzyme, corresponding to 1.5 g/l production of the enzyme | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39350 | - |
MALDI-TOF | Aspergillus oryzae |
40480 | - |
MALDI-TOF | Aspergillus oryzae |
53000 | - |
SDS-PAGE | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the N-terminal analysis of the protein reveals N-terminal processing taking place in the Kex2/furin-type protease cleavage site and removing the first 51 amino acids from the putative N-terminus | Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
AoCO4 is purified with a two-step purification procedure, consisting of cation and anion exchange chromatography | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 catechol + O2 | - |
Aspergillus oryzae | 2 1,2-benzoquinone + 2 H2O | - |
? | |
4-tert-butylcatechol + O2 | - |
Aspergillus oryzae | ? | - |
? | |
aminophenol + O2 | - |
Aspergillus oryzae | ? | - |
? | |
aniline + O2 | enzyme shows also activity after 24 h incubation | Aspergillus oryzae | ? | - |
? | |
caffeic acid + O2 | - |
Aspergillus oryzae | ? | - |
? | |
guaiacol + O2 | - |
Aspergillus oryzae | ? | - |
? | |
p-cresol + O2 | enzyme shows also activity after 24 h incubation | Aspergillus oryzae | ? | - |
? | |
phenol + O2 | enzyme shows also activity after 24 h incubation | Aspergillus oryzae | ? | - |
? | |
tyrosol + O2 | enzyme shows also activity after 24 h incubation | Aspergillus oryzae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AoCO4 | - |
Aspergillus oryzae |
catechol oxidase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-live 20 h at 50°C | Aspergillus oryzae |
60 | - |
enzyme shows thermostability up to 60°C, half-live 2 h at 60°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Aspergillus oryzae |
7 | - |
assay at | Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
cosiderable activity loss below pH 5.0 | Aspergillus oryzae |