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Literature summary for 1.10.3.1 extracted from

  • Kouakou, T.H.; Kouadio, Y.J.; Kouame, P.; Waffo-Teguo, P.; Decendit, A.; Merillon, J.M.
    Purification and biochemical characterization of polyphenol oxidases from embryogenic and nonembryogenic cotton (Gossypium hirsutum L.) cells (2009), Appl. Biochem. Biotechnol., 158, 285-301.
    View publication on PubMed
Search for more literature for 1.10.3.1

Activating Compound

Activating Compound Comment Organism Structure
additional information highest activity is measured using the following buffer composition: sodium phosphate + Triton X-100 (1%) + PVP (0.5%) + EDTA (1.0 mM) + sodium thiosulfate (0.25%) Gossypium hirsutum

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid markedly inhibits PPO Gossypium hirsutum
Ba2+ moderately inhibits PPO Gossypium hirsutum
Cu2+ moderately inhibits PPO Gossypium hirsutum
dithiothreitol markedly inhibits PPO Gossypium hirsutum
FeCl3 markedly inhibits PPO Gossypium hirsutum
SnCl2 markedly inhibits PPO Gossypium hirsutum
Sodium metabisulfite markedly inhibits PPO Gossypium hirsutum
Zn2+ moderately inhibits PPO Gossypium hirsutum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ highly activates PPO Gossypium hirsutum
Mg2+ highly activates PPO Gossypium hirsutum
Mn2+ highly activates PPO Gossypium hirsutum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE Gossypium hirsutum

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Sephadex G-200 column balanced with 0.1 M sodium phosphate buffer (pH 6.5) Gossypium hirsutum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture coker 312 seeds are used. Cotton cell suspensions are obtained from calli derived from hypocotyl segments of plant. Coker 312 cell suspensions show the development of embryogenic structures Gossypium hirsutum
-
cell suspension culture R405-2000 seeds are used. Cotton cell suspensions are obtained from calli derived from hypocotyl segments of plant. R405-2000 cell suspensions does not show the development of embryogenic structures Gossypium hirsutum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 compared to PPO from R405-2000, a nonembryogenic cultivar, PPO from coker 312 (embryogenic cultivar) shows a higher PPO activity increasing markedly form primary culture to third subculture Gossypium hirsutum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2 enzyme shows high affinity to this substrate Gossypium hirsutum 2 1,2-benzoquinone + 2 H2O
-
 ?
2 catechol + O2 enzyme shows highest affinity to this substrate Gossypium hirsutum 2 1,2-benzoquinone + 2 H2O
-
 ?
4-methylcatechol + O2 enzyme shows high affinity to this substrate Gossypium hirsutum ?
-
 ?
4-methylcatechol + O2 enzyme shows highest affinity to this substrate Gossypium hirsutum ?
-
 ?
catechin + O2 enzyme shows moderate affinity to this substrate Gossypium hirsutum ?
-
 ?
dopamine + O2 enzyme shows moderate affinity to this substrate Gossypium hirsutum ?
-
 ?
gallic acid + O2 enzyme shows very low affinity to this substrate Gossypium hirsutum ?
-
 ?
additional information PPO shows activity with biphenols, triphenol but not with monophenols Gossypium hirsutum ?
-
 ?
pyrogallol + O2 enzyme shows low affinity to this substrate Gossypium hirsutum ?
-
 ?
tetramethylbenzidine + O2 enzyme shows low affinity to this substrate Gossypium hirsutum ?
-
 ?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Gossypium hirsutum
PPO from Coker 312, an embryogenic cultivar Gossypium hirsutum
PPO from R405-2000, an nonembryogenic cultivar Gossypium hirsutum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Gossypium hirsutum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 30 60 min Gossypium hirsutum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 optimal pH, highest activity is measured using the following buffer composition: sodium phosphate + Triton X-100 (1%) + PVP (0.5%) + EDTA (1.0 mM) + sodium thiosulfate (0.25%) Gossypium hirsutum
6.5
-
 assay at Gossypium hirsutum
7
-
 optimal pH, highest activity is measured using the following buffer composition: sodium phosphate + Triton X-100 (1%) + PVP (0.5%) + EDTA (1.0 mM) + sodium thiosulfate (0.25%) Gossypium hirsutum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 enzyme retains 95% of its activity Gossypium hirsutum
6 7 enzyme retains 95% of its activity Gossypium hirsutum