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Literature summary for 1.10.3.1 extracted from

  • Kanade, S.R.; Suhas, V.L.; Chandra, N.; Gowda, L.R.
    Functional interaction of diphenols with polyphenol oxidase. Molecular determinants of substrate/inhibitor specificity (2007), FEBS J., 274, 4177-4187.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,3-Dihydroxybenzoic acid
-
Ipomoea batatas
2,3-Dihydroxybenzoic acid
-
Lablab purpureus
3,4,5-Trihydroxybenzoic acid
-
Ipomoea batatas
3,4,5-Trihydroxybenzoic acid
-
Lablab purpureus
3,4-dihydroxybenzoic acid
-
Ipomoea batatas
3,4-dihydroxybenzoic acid
-
Lablab purpureus
4-methylcatechol field bean PPO obeys Michaelis-Menten kinetics and exhibits the phenomenon of inhibition by excess substrate for catechol, 4-methylcatechol and 4-tert-butylcatechol Lablab purpureus
4-tert-butylcatechol field bean PPO obeys Michaelis-Menten kinetics and exhibits the phenomenon of inhibition by excess substrate for catechol, 4-methylcatechol and 4-tert-butylcatechol Lablab purpureus
benzoic acid
-
Ipomoea batatas
benzoic acid
-
Lablab purpureus
catechol field bean PPO obeys Michaelis-Menten kinetics and exhibits the phenomenon of inhibition by excess substrate for catechol, 4-methylcatechol and 4-tert-butylcatechol Lablab purpureus
m-hydroxybenzoic acid
-
Ipomoea batatas
m-hydroxybenzoic acid
-
Lablab purpureus
o-hydroxybenzoic acid
-
Ipomoea batatas
o-hydroxybenzoic acid
-
Lablab purpureus
p-hydroxybenzoic acid
-
Ipomoea batatas
p-hydroxybenzoic acid
-
Lablab purpureus
Phenylthiourea
-
Ipomoea batatas
Phenylthiourea
-
Lablab purpureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
dopamine
-
Lablab purpureus
2.9
-
3,4-dihydroxyphenylacetic acid
-
Lablab purpureus
3.9
-
4-tertiary butylcatechol
-
Ipomoea batatas
4
-
2,3,4-trihydroxybenzoic acid
-
Lablab purpureus
4.1
-
4-tert-butylcatechol
-
Lablab purpureus
4.5
-
4-methylcatechol
-
Lablab purpureus
5.2
-
4-methylcatechol
-
Ipomoea batatas
5.6
-
2,3,4-trihydroxybenzoic acid
-
Ipomoea batatas
6
-
3,4-dihydroxyphenylacetic acid
-
Ipomoea batatas
9
-
catechol
-
Ipomoea batatas
12
-
catechol
-
Lablab purpureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
SDS-PAGE Ipomoea batatas

Organism

Organism UniProt Comment Textmining
Ipomoea batatas Q9ZP19
-
-
Lablab purpureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
99% purity Lablab purpureus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Lablab purpureus
-
tuber
-
Ipomoea batatas
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1574
-
-
Ipomoea batatas
29300
-
-
Lablab purpureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
Ipomoea batatas 2 1,2-benzoquinone + 2 H2O
-
?
2 catechol + O2 field bean PPO obeys Michaelis-Menten kinetics and exhibits the phenomenon of inhibition by excess substrate for catechol, 4-methylcatechol and 4-tert-butylcatechol Lablab purpureus 2 1,2-benzoquinone + 2 H2O
-
?
2 dopamine + O2
-
Lablab purpureus 2 4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
?
2,3,4-trihydroxybenzoic acid + O2
-
Lablab purpureus ?
-
?
2,3,4-trihydroxybenzoic acid + O2
-
Ipomoea batatas ?
-
?
3,4-dihydroxyphenylacetic acid + O2
-
Lablab purpureus ?
-
?
3,4-dihydroxyphenylacetic acid + O2
-
Ipomoea batatas (3,4-dioxocyclohexa-1,5-dien-1-yl)acetic acid + H2O
-
?
4-methylcatechol + O2
-
Ipomoea batatas 4-methyl-1,2-benzoquinone
-
?
4-methylcatechol + O2 field bean PPO obeys Michaelis-Menten kinetics and exhibits the phenomenon of inhibition by excess substrate for catechol, 4-methylcatechol and 4-tert-butylcatechol Lablab purpureus 4-methyl-1,2-benzoquinone
-
?
4-tert-butylcatechol + O2
-
Ipomoea batatas 4-tert-butyl-1,2-benzoquinone + H2O
-
?
4-tert-butylcatechol + O2 field bean PPO obeys Michaelis-Menten kinetics and exhibits the phenomenon of inhibition by excess substrate for catechol, 4-methylcatechol and 4-tert-butylcatechol Lablab purpureus ?
-
?
additional information pyragallol is not oxidized by ibCO Ipomoea batatas ?
-
?

Subunits

Subunits Comment Organism
monomer SDS-PAGE Ipomoea batatas

Synonyms

Synonyms Comment Organism
1,2-benzene: oxygen oxidoreductase
-
Lablab purpureus
1,2-benzene: oxygen oxidoreductase
-
Ipomoea batatas
ibCO sweet potato catechol oxidase Ipomoea batatas
polyphenol oxidase
-
Lablab purpureus
polyphenol oxidase
-
Ipomoea batatas
PPO
-
Lablab purpureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.05
-
2,3,4-trihydroxybenzoic acid
-
Lablab purpureus
4.9
-
dopamine
-
Lablab purpureus
7.48
-
catechol
-
Lablab purpureus
11.48
-
3,4-dihydroxyphenylacetic acid
-
Lablab purpureus
26.66
-
4-methylcatechol
-
Lablab purpureus
48.05
-
4-tert-butylcatechol
-
Lablab purpureus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3
-
3,4,5-Trihydroxybenzoic acid
-
Lablab purpureus
2.3
-
3,4,5-Trihydroxybenzoic acid
-
Ipomoea batatas

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.043
-
-
Ipomoea batatas Phenylthiourea
0.07
-
-
Lablab purpureus Phenylthiourea
1.1
-
-
Lablab purpureus m-hydroxybenzoic acid
1.2
-
-
Lablab purpureus benzoic acid
1.3
-
-
Lablab purpureus p-hydroxybenzoic acid
2.8
-
-
Lablab purpureus 3,4-dihydroxybenzoic acid
3.6
-
-
Lablab purpureus o-hydroxybenzoic acid
3.7
-
-
Lablab purpureus 2,3-Dihydroxybenzoic acid
3.8
-
-
Lablab purpureus 3,4,5-Trihydroxybenzoic acid