BRENDA - Enzyme Database
show all sequences of 1.10.2.1

Partial purification of L-ascorbate:ferricytochrome b5 oxidoreductase from rat liver microsomes

Scherer, G.; Weis, W.; Hoppe-Seyler's Z. Physiol. Chem. 358, S.1499-1503 (1977)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
asolectin
lipid micelles increases activity about 2fold
Rattus norvegicus
lecithin
lipid micelles increases activity about 2fold
Rattus norvegicus
General Stability
General Stability
Organism
enzymes loses its activity during isoelectric focusing
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Rattus norvegicus
16020
-
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-(+)-ascorbate + ferricytochrome b5
Rattus norvegicus
-
monodehydro-L(+)-ascorbate + ferrocytochrome b5
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
rat, Wistar AF/Han
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.5
-
microsomes after CM-cellulose-chromatography, ultrafiltration and dialysis P2
Rattus norvegicus
Storage Stability
Storage Stability
Organism
0°C, enzymatic activity tolerates ampholine over a 24 h period
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-(+)-ascorbate + ferricytochrome b5
-
389973
Rattus norvegicus
monodehydro-L(+)-ascorbate + ferrocytochrome b5
-
-
-
?
L-ascorbate + ferricytochrome b5
-
389973
Rattus norvegicus
monodehydro-L-ascorbate + ferrocytochrome b5
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
-
enriched enzyme
Rattus norvegicus
7.2
7.4
in microsomes
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
asolectin
lipid micelles increases activity about 2fold
Rattus norvegicus
lecithin
lipid micelles increases activity about 2fold
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
enzymes loses its activity during isoelectric focusing
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Rattus norvegicus
16020
-
microsome
-
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-(+)-ascorbate + ferricytochrome b5
Rattus norvegicus
-
monodehydro-L(+)-ascorbate + ferrocytochrome b5
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.5
-
microsomes after CM-cellulose-chromatography, ultrafiltration and dialysis P2
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
0°C, enzymatic activity tolerates ampholine over a 24 h period
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-(+)-ascorbate + ferricytochrome b5
-
389973
Rattus norvegicus
monodehydro-L(+)-ascorbate + ferrocytochrome b5
-
-
-
?
L-ascorbate + ferricytochrome b5
-
389973
Rattus norvegicus
monodehydro-L-ascorbate + ferrocytochrome b5
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.9
-
enriched enzyme
Rattus norvegicus
7.2
7.4
in microsomes
Rattus norvegicus
Other publictions for EC 1.10.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389975
Knoepfel
Characterization of a cytochro ...
Mus musculus, Oryctolagus cuniculus
Biochem. Biophys. Res. Commun.
291
220-225
2002
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1
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1
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2
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389974
Scherer
-
Participation of L-ascorbate:f ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
359
S.1527-1530
1978
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1
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1
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1
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389973
Scherer
Partial purification of L-asco ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
358
S.1499-1503
1977
2
-
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-
-
1
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2
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1
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1
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1
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1
1
2
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1
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2
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1
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1
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1
1
1
2
-
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-
-
-
2
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389972
Wolf
Discrimination between ascorba ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
72
190-194
1976
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-
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1
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1
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1
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1
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1
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389970
Scherer
Trägergebundenes Cytochrom b5 ...
Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
355
S.1350-1354
1974
-
-
-
-
-
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2
1
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1
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1
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1
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1
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2
1
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1
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1
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1
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389971
Weber
Monodehydro-L(+)-ascorbat redu ...
Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
355
S.595-599
1974
-
-
-
-
-
-
-
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2
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1
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1
1
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1
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2
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1
1
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1
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-
389969
Weber
Unterschiedliche Cytochrom-b5- ...
Oryctolagus cuniculus, Rattus norvegicus, Sus scrofa
Hoppe-Seyler's Z. Physiol. Chem.
354
S.1277-1284
1973
-
-
-
-
-
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-
17
3
-
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1
-
3
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2
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4
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3
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17
3
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1
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2
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4
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3
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389968
Weber
Praeparative Untersuchungen an ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
353
S.1415-1419
1972
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1
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389967
Everling
Kinetische Untersuchungen an e ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
350
S.1485-1492
1969
-
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-
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1
1
1
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1
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2
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1
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2
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1
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1
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