BRENDA - Enzyme Database
show all sequences of 1.1.99.B9

One-pot, single-step deracemization of 2-hydroxyacids by tandem biocatalytic oxidation and reduction

Xue, Y.; Zheng, Y.; Zhang, Y.; Sun, J.; Liu, Z.; Shen, Y.; Chem. Commun. (Camb.) 49, 10706-10708 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
coupling of asymmetric oxidation of racemic 2-hydroxyacids with the opposite stereoselective reduction of 2-ketoacids for the stereoselective oxidoreductive deracemization to (R)-2-hydroxyacids, using a one-pot biocatalysis by resting cells of Saccharomyces cerevisiae strain ZJB5074 and Pseudomonas aeruginosa CCTCC M 2011394. Substituted racemic hydroxy(phenyl)acetic acids are converted to (R)-2-hydroxy-2-phenyl acetic acids with 55-98% conversion rate and 99.9% enantiomeric excess
Pseudomonas aeruginosa
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa CCTCC M2011394
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
-
Pseudomonas aeruginosa
Application (protein specific)
Application
Commentary
Organism
synthesis
coupling of asymmetric oxidation of racemic 2-hydroxyacids with the opposite stereoselective reduction of 2-ketoacids for the stereoselective oxidoreductive deracemization to (R)-2-hydroxyacids, using a one-pot biocatalysis by resting cells of Saccharomyces cerevisiae strain ZJB5074 and Pseudomonas aeruginosa CCTCC M 2011394. Substituted racemic hydroxy(phenyl)acetic acids are converted to (R)-2-hydroxy-2-phenyl acetic acids with 55-98% conversion rate and 99.9% enantiomeric excess
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
-
Pseudomonas aeruginosa
Other publictions for EC 1.1.99.B9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740168
Xue
Highly enantioselective oxidat ...
Sinorhizobium sp., Sinorhizobium sp. ZJB1101
Biores. Technol.
132
391-394
2013
-
-
-
-
-
-
-
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740300
Xue
One-pot, single-step deracemiz ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa CCTCC M2011394
Chem. Commun. (Camb.)
49
10706-10708
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
1
-
1
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-
-
-
740783
Xue
Concurrent obtaining of aromat ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa ZJB1125
J. Biotechnol.
167
271-278
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
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-
-
-
-
-
-
15
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
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-
-
-
-
-
-
-
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-
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15
-
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-
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-
-
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1
-
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1
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