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Literature summary for 1.1.99.B3 extracted from

  • Vuong, T.V.; Vesterinen, A.H.; Foumani, M.; Juvonen, M.; Seppaelae, J.; Tenkanen, M.; Master, E.R.
    Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictum and variants with reduced substrate inhibition (2013), Biotechnol. Biofuels, 6, 148.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A38V the mutation significantly increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum
A38V/S388N the mutation increases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum
E247A the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum
Q353A the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum
Q353N the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum
Q384A the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum
Q384N the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum
W351A the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum
W351A the mutation increases Km values by up to 2 orders of magnitude while also increasing kcat up to 3fold on cello- and xylo-oligosaccharides and showing no substrate inhibition compared to the wild type enzyme Sarocladium strictum
Y72A the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum
Y72F the mutation decreases the kcat and catalytic efficiency of the enzyme on oligosaccharides compared to the wild type enzyme Sarocladium strictum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
xylohexaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.039
-
cellobiose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.04
-
cellobiose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.05
-
cellotriose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.05
-
xylopentaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.05
-
cellotriose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.05
-
xylobiose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.05
-
xylopentaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.05
-
cellobiose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.052
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.057
-
xylobiose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.057
-
cellopentaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.059
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.06
-
cellopentaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.06
-
xylohexaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.06
-
cellotetraose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.067
-
xylotetraose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.07
-
cellotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.07
-
xylotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.078
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.08
-
xylotriose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.08
-
cellohexaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.08
-
xylotriose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.08
-
cellohexaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.08
-
cellotriose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.09
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.09
-
cellotetraose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.09
-
cellopentaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.099
-
xylobiose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.1
-
xylotriose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.1
-
cellohexaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.1
-
xylohexaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.104
-
xylopentaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.11
-
xylotetraose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.3
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
12
-
D-glucose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
15
-
D-glucose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
15
-
D-glucose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
118
-
D-xylose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
118
-
D-xylose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
129
-
D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum

Organism

Organism UniProt Comment Textmining
Sarocladium strictum D7PF15
-
-
Sarocladium strictum CBS 346.70 D7PF15
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Sarocladium strictum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
Sarocladium strictum 4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
?
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
Sarocladium strictum CBS 346.70 4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
Sarocladium strictum alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
?
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
Sarocladium strictum CBS 346.70 alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
Sarocladium strictum alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
?
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose + O2
-
Sarocladium strictum CBS 346.70 alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylono-1,5-lactone + H2O2
-
?
cellobiose + O2
-
Sarocladium strictum cellobiono-1,5-lactone + H2O2
-
?
cellobiose + O2
-
Sarocladium strictum CBS 346.70 cellobiono-1,5-lactone + H2O2
-
?
cellohexaose + O2
-
Sarocladium strictum cellohexaono-1,5-lactone + H2O2
-
?
cellopentaose + O2
-
Sarocladium strictum cellopentaono-1,5-lactone + H2O2
-
?
cellotetraose + O2
-
Sarocladium strictum cellotetraono-1,5-lactone + H2O2
-
?
cellotriose + O2
-
Sarocladium strictum cellotriono-1,5-lactone + H2O2
-
?
D-glucose + O2
-
Sarocladium strictum D-glucono-1,5-lactone + H2O2
-
?
D-glucose + O2
-
Sarocladium strictum CBS 346.70 D-glucono-1,5-lactone + H2O2
-
?
D-xylose + O2
-
Sarocladium strictum D-xylono-1,5-lactone + H2O2
-
?
xylobiose + O2 highest activity Sarocladium strictum xylobiono-1,5-lactone + H2O2
-
?
xylohexaose + O2
-
Sarocladium strictum xylohexaono-1,5-lactone + H2O2
-
?
xylopentaose + O2
-
Sarocladium strictum xylopentaono-1,5-lactone + H2O2
-
?
xylotetraose + O2
-
Sarocladium strictum xylotetraono-1,5-lactone + H2O2
-
?
xylotriose + O2
-
Sarocladium strictum xylotriono-1,5-lactone + H2O2
-
?

Synonyms

Synonyms Comment Organism
gluco-oligosaccharide oxidase
-
Sarocladium strictum
GOOX
-
Sarocladium strictum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
D-xylose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
6
-
D-glucose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
6.2
-
cellobiose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
6.3
-
D-glucose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
6.6
-
cellohexaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
6.9
-
cellotriose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
7
-
cellobiose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
7.2
-
cellohexaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
7.3
-
cellotriose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
7.4
-
D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
7.5
-
xylobiose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
7.6
-
cellopentaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
7.8
-
cellotetraose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
7.8
-
xylohexaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
8.1
-
xylotetraose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
8.3
-
cellopentaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
8.6
-
xylohexaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
8.7
-
xylobiose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
8.7
-
xylotriose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
8.8
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
8.8
-
xylopentaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
9
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
9.7
-
xylotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
9.9
-
cellotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
10.3
-
xylotriose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
10.4
-
xylopentaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
10.4
-
D-glucose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
11
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
12
-
cellobiose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
12.3
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
12.4
-
D-xylose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
12.7
-
cellopentaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
12.8
-
cellotetraose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
13.2
-
cellohexaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
13.5
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
13.7
-
cellotriose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
14.5
-
xylotriose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
14.8
-
xylobiose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
15.2
-
xylopentaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
15.2
-
xylohexaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
16
-
xylotetraose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Sarocladium strictum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
D-xylose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.06
-
D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.11
-
D-xylose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
0.4
-
D-glucose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
0.42
-
D-glucose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
0.83
-
D-glucose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
36.5
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
66.7
-
cellohexaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
73.3
-
xylotetraose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
73.3
-
xylohexaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
75
-
xylobiose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
78.3
-
cellopentaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
85
-
xylotriose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
85
-
xylopentaose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
86.7
-
cellotetraose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
91.7
-
cellohexaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
93.3
-
cellotriose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
115
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
116.7
-
cellobiose mutant enzyme A38V/S388N, at pH 8.0 and 37°C Sarocladium strictum
135
-
xylohexaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
136.7
-
xylotriose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
136.7
-
xylotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
143.3
-
cellotriose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
145
-
cellopentaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
148.3
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
150
-
cellotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
150
-
cellohexaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
150
-
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
151.7
-
xylobiose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
156.7
-
cellobiose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
183.3
-
xylotriose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
196.7
-
xylopentaose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum
216.7
-
cellopentaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
233.3
-
cellotetraose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
233.3
-
xylotetraose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
233.3
-
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
250
-
cellotriose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
283.3
-
xylopentaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
300
-
xylobiose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
300
-
xylohexaose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum
306.7
-
cellobiose mutant enzyme A38V, at pH 8.0 and 37°C Sarocladium strictum