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Literature summary for 1.1.99.B3 extracted from

  • Huang, C.H.; Winkler, A.; Chen, C.L.; Lai, W.L.; Tsai, Y.C.; Macheroux, P.; Liaw, S.H.
    Functional roles of the 6-S-cysteinyl, 8alpha-N1-histidyl FAD in glucooligosaccharide oxidase from Acremonium strictum (2008), J. Biol. Chem., 283, 30990-30996.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Sarocladium strictum

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Sarocladium strictum

Protein Variants

Protein Variants Comment Organism
C130A FAD binding site, FAD covalently attached Sarocladium strictum
H70A FAD binding site, FAD covalently attached Sarocladium strictum
H70A/C130A no activity, lack of essential FAD cofactor Sarocladium strictum
Y310A larger amount of carbohydrates Sarocladium strictum

General Stability

General Stability Organism
and wild-type proteins are 1.9 M, 2.4 M, 2.6 M, and 3.2 M, respectively. Sarocladium strictum
and wild-type proteins are 48°C, 52°C, 46°C, and 56°C, respectively. Sarocladium strictum
The melting temperatures for the H70A, C130A, H70A/C130A, Sarocladium strictum
The [guanidine HCl]1/2 values for the H70A, C130A, H70A/C130A, Sarocladium strictum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
cellobiose wild type protein Sarocladium strictum
0.22
-
cellobiose H70A mutant protein Sarocladium strictum
0.4
-
cellobiose C130A mutant protein Sarocladium strictum

Organism

Organism UniProt Comment Textmining
Sarocladium strictum Q6PW77
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Sarocladium strictum

Purification (Commentary)

Purification (Comment) Organism
-
Sarocladium strictum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cello-oligosaccharide + acceptor
-
Sarocladium strictum ?
-
?
cellobiose + acceptor
-
Sarocladium strictum ?
-
?
D-glucose + acceptor
-
Sarocladium strictum ?
-
?
lactose + acceptor
-
Sarocladium strictum ?
-
?
malto-oligosaccharide + acceptor
-
Sarocladium strictum ?
-
?
maltose + acceptor
-
Sarocladium strictum ?
-
?

Synonyms

Synonyms Comment Organism
glucooligosaccharide oxidase
-
Sarocladium strictum
GOOX
-
Sarocladium strictum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
cellobiose H70A mutant protein Sarocladium strictum
0.58
-
cellobiose C130A mutant protein Sarocladium strictum
11.9
-
cellobiose wild type protein Sarocladium strictum

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound Sarocladium strictum