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Literature summary for 1.1.99.9 extracted from

  • Jong, Y.J.; Nelson, M.J.K.; Snell, E.E.
    Enzymes of vitamin B6 degradation. Purification and properties of pyridoxine 5-dehydrogenase (oxidase) (1986), J. Biol. Chem., 261, 15102-15105.
    View publication on PubMed
Search for more literature for 1.1.99.9

Inhibitors

Inhibitors Comment Organism Structure
4-pyridoxic acid
-
 Arthrobacter sp.
Cu2+
-
 Arthrobacter sp.
Dithionitrobenzoic acid
-
 Arthrobacter sp.
Hg2+
-
 Arthrobacter sp.
Isopyridoxal
-
 Arthrobacter sp.
N-ethylmaleimide
-
 Arthrobacter sp.
Ni2+
-
 Arthrobacter sp.
p-chloromercuribenzoate
-
 Arthrobacter sp.
pyridoxal
-
 Arthrobacter sp.
pyridoxamine
-
 Arthrobacter sp.
sulfhydryl reagents
-
 Arthrobacter sp.
Zn2+
-
 Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 pyridoxine
-
 Arthrobacter sp.
0.28
-
 O2
-
 Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Arthrobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58600
-
 2 * 58600, SDS-PAGE Arthrobacter sp.
112000
-
 gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxine + acceptor Arthrobacter sp. utilization of pyridoxine as sole source of carbon and nitrogen isopyridoxal + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 Cr-7, inducible enzyme
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.9
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-deoxypyridoxine + acceptor 18% of activity with pyridoxine Arthrobacter sp. 3-hydroxy-2-methylpyridine-5-carboxaldehyde + reduced acceptor
-
 ?
pyridoxal + acceptor 29% of activity with pyridoxine Arthrobacter sp. 3-hydroxy-2-methylpyridine-4,5-dicarbaldehyde + reduced acceptor
-
 ?
pyridoxamine + acceptor 27% of activity with pyridoxine Arthrobacter sp. 4-aminomethyl-5-formyl-2-methylpyridin-3-ol + reduced acceptor
-
 ?
pyridoxine + acceptor NAD+, NADP+ Arthrobacter sp. isopyridoxal + reduced acceptor
-
 ?
pyridoxine + acceptor O2 or quinones Arthrobacter sp. isopyridoxal + reduced acceptor
-
 ?
pyridoxine + acceptor acceptor: 2,6-dichlorophenolindophenol Arthrobacter sp. isopyridoxal + reduced acceptor
-
 ?
pyridoxine + acceptor utilization of pyridoxine as sole source of carbon and nitrogen Arthrobacter sp. isopyridoxal + reduced acceptor
-
 ?
pyridoxine + O2
-
 Arthrobacter sp. isopyridoxal + H2O2
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 58600, SDS-PAGE Arthrobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22
-
 pyridoxine acceptor O2 Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.3 pyridoxine + O2, Tris buffer, phosphate buffer Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.4 9.5 activity at pH 9.5 in Tris is about 70% of that at pH 7.3 while activities at pH 6.4 and pH 6.0 in phosphate buffer are about 75% and 0% respectively of that at pH 7.3 Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein, FAD-dependent Arthrobacter sp.