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Literature summary for 1.1.99.7 extracted from

  • Allen, S.H.G.
    Malate-lactate transhydrogenase from Micrococcus lactilyticus (1969), Methods Enzymol., 13, 262-269.
No PubMed abstract available

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion required Veillonella parvula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99000 100000 sucrose density gradient studies Veillonella parvula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + oxaloacetate Veillonella parvula first step in fermentation of lactate malate + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Veillonella parvula
-
formerly named as Micrococcus lactilyticus
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, DEAE-Sephadex Veillonella parvula

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
117
-
-
Veillonella parvula

Storage Stability

Storage Stability Organism
-15°C, as ammonium sulfate suspension, about 10-20 mg of protein per ml, 40% loss of activity after 1 year Veillonella parvula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + oxaloacetate 50% higher specific activity with malate and pyruvate as substrates Veillonella parvula malate + pyruvate
-
?
(S)-lactate + oxaloacetate first step in fermentation of lactate Veillonella parvula malate + pyruvate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Veillonella parvula

pH Range

pH Minimum pH Maximum Comment Organism
6.3 9.5 about 50% of maximal activity at pH 6.3 and 9.5 Veillonella parvula