Literature summary for 1.1.99.7 extracted from
Allen, S.H.G.
Malate-lactate transhydrogenase from Micrococcus lactilyticus (1969), Methods Enzymol., 13, 262-269.
No PubMed abstract available
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
additional information |
no metal ion required |
Veillonella parvula |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
99000 |
100000 |
sucrose density gradient studies |
Veillonella parvula |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
(S)-lactate + oxaloacetate |
Veillonella parvula |
first step in fermentation of lactate |
malate + pyruvate |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Veillonella parvula |
- |
formerly named as Micrococcus lactilyticus |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
DEAE-cellulose, DEAE-Sephadex |
Veillonella parvula |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
117 |
- |
- |
Veillonella parvula |
Storage Stability
Storage Stability |
Organism |
-15°C, as ammonium sulfate suspension, about 10-20 mg of protein per ml, 40% loss of activity after 1 year |
Veillonella parvula |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
(S)-lactate + oxaloacetate |
50% higher specific activity with malate and pyruvate as substrates |
Veillonella parvula |
malate + pyruvate |
- |
? |
|
(S)-lactate + oxaloacetate |
first step in fermentation of lactate |
Veillonella parvula |
malate + pyruvate |
- |
? |
|
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7.5 |
8.5 |
- |
Veillonella parvula |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
6.3 |
9.5 |
about 50% of maximal activity at pH 6.3 and 9.5 |
Veillonella parvula |