Application | Comment | Organism |
---|---|---|
analysis | construction of a D-Lactate electrochemical biosensor. The electrode for detection of D-lactate is prepared by immobilizing dye-linked D-LDH and multi-walled carbon nanotube within Nafion membrane. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days | Candidatus Caldarchaeum subterraneum |
analysis | D-lactate content is of great interest for food analysis. A D-lactate sensing system using a thermostable dye-linked D-lactate dehydrogenase (Dye-DLDH) is developed. The electrode for detection of D-lactate is prepared by immobilizing the thermostable Dye-DLDH and multi-walled carbon nanotube (MWCNT) within Nafion membrane. The electrocatalytic response of the electrode is clearly observed upon exposure to D-lactate. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days | Candidatus Caldarchaeum subterraneum |
diagnostics | D-lactate content is of great interest for clinical diagnosis. A D-lactate sensing system using a thermostable dye-linked D-lactate dehydrogenase (Dye-DLDH) is developed. The electrode for detection of D-lactate is prepared by immobilizing the thermostable Dye-DLDH and multi-walled carbon nanotube (MWCNT) within Nafion membrane. The electrocatalytic response of the electrode is clearly observed upon exposure to D-lactate. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days | Candidatus Caldarchaeum subterraneum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Candidatus Caldarchaeum subterraneum |
overexpressed in Escherichia coli | Candidatus Caldarchaeum subterraneum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.605 | - |
(R)-lactate | pH 7.0, 60°C, electron acceptor: oxidized 2,6-dichlorophenolindophenol | Candidatus Caldarchaeum subterraneum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
114000 | - |
gel filtration | Candidatus Caldarchaeum subterraneum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + acceptor | Candidatus Caldarchaeum subterraneum | - |
pyruvate + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candidatus Caldarchaeum subterraneum | E6N788 | - |
- |
Candidatus Caldarchaeum subterraneum | E6N788 | cf. EC 1.1.3.15 | - |
Purification (Comment) | Organism |
---|---|
- |
Candidatus Caldarchaeum subterraneum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.18 | - |
pH 7.0, 60°C | Candidatus Caldarchaeum subterraneum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-hydroxybutanoate + 2,6-dichlorophenolindophenol | preferred substrate | Candidatus Caldarchaeum subterraneum | 2-oxobutanoate + reduced 2,6-dichlorophenolindophenol | - |
? | |
(R)-2-hydroxybutyrate + oxidized 2,6-dichlorophenolindophenol | relative activity is 46% compared to D-lactate | Candidatus Caldarchaeum subterraneum | 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol | - |
? | |
(R)-lactate + 2,6-dichlorophenolindophenol | preferred substrate | Candidatus Caldarchaeum subterraneum | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
(R)-lactate + acceptor | - |
Candidatus Caldarchaeum subterraneum | pyruvate + reduced acceptor | - |
? | |
(R)-lactate + ferricyanide | - |
Candidatus Caldarchaeum subterraneum | pyruvate + ferrocyanide + H+ | - |
? | |
(R)-lactate + oxidized 2,6-dichlorophenolindophenol | (R)-lactate is the most preferred substrate, followed by (R)-2-hydroxybutyrate. D-Alanine, pyruvate, D-glucose, L-alanine, ethanol and L-lactate are inert as electron donors. Benzyl viologen and methylene blue are inert as electron acceptors | Candidatus Caldarchaeum subterraneum | pyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
(R)-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
Candidatus Caldarchaeum subterraneum | pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide | - |
? | |
additional information | no substrates: D-alanine, pyruvate, D-glucose, L-alanine, ethanol and L-lactate | Candidatus Caldarchaeum subterraneum | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 51000, SDS-PAGE | Candidatus Caldarchaeum subterraneum |
homodimer | 2 * 51000, SDS-PAGE | Candidatus Caldarchaeum subterraneum |
Synonyms | Comment | Organism |
---|---|---|
CSUB_C1080 | - |
Candidatus Caldarchaeum subterraneum |
dye-DLDH | - |
Candidatus Caldarchaeum subterraneum |
dye-linked D-lactate dehydrogenase | - |
Candidatus Caldarchaeum subterraneum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, pH 6.0 or pH 8.0, no loss of activity | Candidatus Caldarchaeum subterraneum |
70 | - |
10 min, no loss of acitvity | Candidatus Caldarchaeum subterraneum |
70 | - |
retains full activity after incubation for 10 min at 70°C | Candidatus Caldarchaeum subterraneum |
80 | - |
10 min, 30% loss of activity | Candidatus Caldarchaeum subterraneum |
90 | - |
10 min, 90% loss of activity | Candidatus Caldarchaeum subterraneum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Candidatus Caldarchaeum subterraneum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
30 min, 50°C, 40% loss of activity | Candidatus Caldarchaeum subterraneum |
6 | - |
30 min, 50°C, no loss of activity | Candidatus Caldarchaeum subterraneum |
8 | - |
30 min, 50°C, no loss of activity | Candidatus Caldarchaeum subterraneum |
9 | - |
30 min, 50°C, 40% loss of activity | Candidatus Caldarchaeum subterraneum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Candidatus Caldarchaeum subterraneum | |
FAD | cofactor | Candidatus Caldarchaeum subterraneum |