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Literature summary for 1.1.99.6 extracted from

  • Satomura, T.; Hayashi, J.; Sakamoto, H.; Nunoura, T.; Takaki, Y.; Takai, K.; Takami, H.; Ohshima, T.; Sakuraba, H.; Suye, S.I.
    D-Lactate electrochemical biosensor prepared by immobilization of thermostabledye-linked D-lactate dehydrogenase from Candidatus Caldiarchaeum subterraneum (2018), J. Biosci. Bioeng., 126, 425-430 .
    View publication on PubMed

Application

Application Comment Organism
analysis construction of a D-Lactate electrochemical biosensor. The electrode for detection of D-lactate is prepared by immobilizing dye-linked D-LDH and multi-walled carbon nanotube within Nafion membrane. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days Candidatus Caldarchaeum subterraneum
analysis D-lactate content is of great interest for food analysis. A D-lactate sensing system using a thermostable dye-linked D-lactate dehydrogenase (Dye-DLDH) is developed. The electrode for detection of D-lactate is prepared by immobilizing the thermostable Dye-DLDH and multi-walled carbon nanotube (MWCNT) within Nafion membrane. The electrocatalytic response of the electrode is clearly observed upon exposure to D-lactate. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days Candidatus Caldarchaeum subterraneum
diagnostics D-lactate content is of great interest for clinical diagnosis. A D-lactate sensing system using a thermostable dye-linked D-lactate dehydrogenase (Dye-DLDH) is developed. The electrode for detection of D-lactate is prepared by immobilizing the thermostable Dye-DLDH and multi-walled carbon nanotube (MWCNT) within Nafion membrane. The electrocatalytic response of the electrode is clearly observed upon exposure to D-lactate. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days Candidatus Caldarchaeum subterraneum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Candidatus Caldarchaeum subterraneum
overexpressed in Escherichia coli Candidatus Caldarchaeum subterraneum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.605
-
(R)-lactate pH 7.0, 60°C, electron acceptor: oxidized 2,6-dichlorophenolindophenol Candidatus Caldarchaeum subterraneum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
114000
-
gel filtration Candidatus Caldarchaeum subterraneum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + acceptor Candidatus Caldarchaeum subterraneum
-
pyruvate + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Candidatus Caldarchaeum subterraneum E6N788
-
-
Candidatus Caldarchaeum subterraneum E6N788 cf. EC 1.1.3.15
-

Purification (Commentary)

Purification (Comment) Organism
-
Candidatus Caldarchaeum subterraneum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.18
-
pH 7.0, 60°C Candidatus Caldarchaeum subterraneum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-hydroxybutanoate + 2,6-dichlorophenolindophenol preferred substrate Candidatus Caldarchaeum subterraneum 2-oxobutanoate + reduced 2,6-dichlorophenolindophenol
-
?
(R)-2-hydroxybutyrate + oxidized 2,6-dichlorophenolindophenol relative activity is 46% compared to D-lactate Candidatus Caldarchaeum subterraneum 2-oxobutyrate + reduced 2,6-dichlorophenolindophenol
-
?
(R)-lactate + 2,6-dichlorophenolindophenol preferred substrate Candidatus Caldarchaeum subterraneum pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
(R)-lactate + acceptor
-
Candidatus Caldarchaeum subterraneum pyruvate + reduced acceptor
-
?
(R)-lactate + ferricyanide
-
Candidatus Caldarchaeum subterraneum pyruvate + ferrocyanide + H+
-
?
(R)-lactate + oxidized 2,6-dichlorophenolindophenol (R)-lactate is the most preferred substrate, followed by (R)-2-hydroxybutyrate. D-Alanine, pyruvate, D-glucose, L-alanine, ethanol and L-lactate are inert as electron donors. Benzyl viologen and methylene blue are inert as electron acceptors Candidatus Caldarchaeum subterraneum pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
(R)-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
Candidatus Caldarchaeum subterraneum pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
-
?
additional information no substrates: D-alanine, pyruvate, D-glucose, L-alanine, ethanol and L-lactate Candidatus Caldarchaeum subterraneum ?
-
-

Subunits

Subunits Comment Organism
dimer 2 * 51000, SDS-PAGE Candidatus Caldarchaeum subterraneum
homodimer 2 * 51000, SDS-PAGE Candidatus Caldarchaeum subterraneum

Synonyms

Synonyms Comment Organism
CSUB_C1080
-
Candidatus Caldarchaeum subterraneum
dye-DLDH
-
Candidatus Caldarchaeum subterraneum
dye-linked D-lactate dehydrogenase
-
Candidatus Caldarchaeum subterraneum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
30 min, pH 6.0 or pH 8.0, no loss of activity Candidatus Caldarchaeum subterraneum
70
-
10 min, no loss of acitvity Candidatus Caldarchaeum subterraneum
70
-
retains full activity after incubation for 10 min at 70°C Candidatus Caldarchaeum subterraneum
80
-
10 min, 30% loss of activity Candidatus Caldarchaeum subterraneum
90
-
10 min, 90% loss of activity Candidatus Caldarchaeum subterraneum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Candidatus Caldarchaeum subterraneum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
30 min, 50°C, 40% loss of activity Candidatus Caldarchaeum subterraneum
6
-
30 min, 50°C, no loss of activity Candidatus Caldarchaeum subterraneum
8
-
30 min, 50°C, no loss of activity Candidatus Caldarchaeum subterraneum
9
-
30 min, 50°C, 40% loss of activity Candidatus Caldarchaeum subterraneum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Candidatus Caldarchaeum subterraneum
FAD cofactor Candidatus Caldarchaeum subterraneum