Any feedback?
Literature summary for 1.1.99.6 extracted from
- The (2R)-hydroxycarboxylate-viologen-oxidoreductase from Proteus vulgaris is a molybdenum-containing iron-sulphur protein (1994), Eur. J. Biochem., 222, 1025-1032.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCN | 5 mM, 50% inhibition after 7 min, complete inhibition after 1 h | Proteus vulgaris |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | molybdenum containing enzyme | Proteus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
x * 80000, probably octamer, SDS-PAGE | Proteus vulgaris |
| 180000 | 600000 | gel filtration, native PAGE | Proteus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Proteus vulgaris | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxygen-sensitive enzyme, has to be handled in the presence of detergent and absence of oxygen, if stirred under air at room temperatures 50% activity are lost within 30 min, the residual activity has a half-life of 10 h | Proteus vulgaris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, hydroxyapatite, gel filtration | Proteus vulgaris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 270 | - |
80000 Da form of the enzyme | Proteus vulgaris |
| 1800 | - |
600000 Da form of the enzyme | Proteus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + acceptor | - |
Proteus vulgaris | pyruvate + reduced acceptor | - |
r | |
| D-gluconate + acceptor | - |
Proteus vulgaris | 2-oxo-gluconate + reduced acceptor | - |
r | |
| D-glucose 6-phosphate + acceptor | - |
Proteus vulgaris | ? | - |
r | |
| N-acetylneuraminate + acceptor | - |
Proteus vulgaris | ? | - |
r | |
| phenylpyruvate + reduced acceptor | - |
Proteus vulgaris | phenyl-(R)-lactate + acceptor | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 80000, probably octamer, SDS-PAGE | Proteus vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| anthraquinone-2,6-disulfonate | artificial electron acceptor | Proteus vulgaris | |
| molybdopterin mononucleotide | - |
Proteus vulgaris | |
| additional information | molybenum-containing Fe-S-enzyme without an additional prosthetic group such as flavin or cytochrome c, enzyme contains one molybdenum, four iron and four sulfur atoms per subunit | Proteus vulgaris |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
