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Literature summary for 1.1.99.42 extracted from

  • Yagi, T.; Kishore, G.M.; Snell, E.E.
    The bacterial oxidation of vitamin B6. 4-Pyridoxic acid dehydrogenase a membrane-bound enzyme from Pseudomonas MA-1 (1983), J. Biol. Chem., 258, 9419-9425 .
    View publication on PubMed
Search for more literature for 1.1.99.42

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: cyanide Pseudomonas sp. MA-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0084
-
 4-pyridoxate electron acceptor phenazine methosulfate, pH 8.0, 25°C Pseudomonas sp. MA-1
0.0093
-
 4-pyridoxate electron acceptor 2,6-dichloroindophenol, pH 8.0, 25°C Pseudomonas sp. MA-1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 undenatured preparations aggregate readily, leading to mass values of 148,000, 470,000, and more than 670000 obtained by density gradient centrifugation or by gel filtration Pseudomonas sp. MA-1
61000
-
 and 63000, PAGE Pseudomonas sp. MA-1
63000
-
 and 61000, PAGE Pseudomonas sp. MA-1

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. MA-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from Pseudomonas MA-1 grown with pyridoxine as a sole source of carbon and nitrogen Pseudomonas sp. MA-1

Storage Stability

Storage Stability Organism
4°C, half-life 3 days in absence of glycerol. In presence of glycerol 0-20% of the activity is lost in 1 week Pseudomonas sp. MA-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-pyridoxate + 2,6-dichloroindophenol
-
 Pseudomonas sp. MA-1 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol
-
 ?
4-pyridoxate + phenazine methosulfate
-
 Pseudomonas sp. MA-1 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced phenazine methosulfate
-
 ?
additional information 2,6-dichloroindophenol, phenazine methosulfate, and menadione are effective electron acceptors. Ubiquinones are less active, while NAD, FAD, and O2 are inactive. In membrane fractions, oxygen supports 4-pyridoxic acid oxidation via a CN--sensitive electron transport chain Pseudomonas sp. MA-1 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas sp. MA-1

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas sp. MA-1