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Literature summary for 1.1.99.42 extracted from

  • Yokochi, N.; Yoshikane, Y.; Matsumoto, S.; Fujisawa, M.; Ohnishi, K.; Yagi, T.
    Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase (2009), J. Biochem., 145, 493-503 .
    View publication on PubMed
Search for more literature for 1.1.99.42

Protein Variants

Protein Variants Comment Organism
E149Q mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH Mesorhizobium loti
H137L almost complete loss of activity Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0124
-
 NADH wild-type, pH 8.0, 30°C Mesorhizobium loti
0.02
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 8.0, 30°C Mesorhizobium loti
0.0249
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate wild-type, pH 8.0, 30°C Mesorhizobium loti
0.026
-
 NADH mutant E149Q, pH 8.0, 30°C Mesorhizobium loti
0.03
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 5.0, 30°C Mesorhizobium loti
0.033
-
 NADH mutant E149Q, pH 5.0, 30°C Mesorhizobium loti

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59100
-
 gel filtration Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti Q988H5 enzyme additionally catalyzes the reaction of EC 1.2.1.100
-
Mesorhizobium loti MAFF303099 Q988H5 enzyme additionally catalyzes the reaction of EC 1.2.1.100
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+
-
 Mesorhizobium loti 4-pyridoxate + NAD+
-
 ?
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+
-
 Mesorhizobium loti MAFF303099 4-pyridoxate + NAD+
-
 ?
additional information enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, i.e. reaction of EC 1.2.1.100, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction Mesorhizobium loti ?
-
 ?
additional information enzyme catalyzes practically irreversible oxidation of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NAD+ to 3-hydroxy-2-methyl-pyridine 4, 5-dicarboxylic acid, i.e. reaction of EC 1.2.1.100, and practically irreversible reduction of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate by NADH to 4-pyridoxic acid. When the enzyme reaction is started with the combination of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NAD+ or that of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate and NADH, 3-hydroxy-2-methylpyridine-4,5-dicarboxylate and 4-pyridoxic acid are produced in an almost equimolar ratio throughout the reaction Mesorhizobium loti MAFF303099 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38900, SDS-PAGE, 2 * 33060, calculated from sequence, 2 * 33121, TOF-MS Mesorhizobium loti

Synonyms

Synonyms Comment Organism
mlr6739
-
 Mesorhizobium loti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
78
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 8.0, 30°C Mesorhizobium loti
217
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate wild-type, pH 8.0, 30°C Mesorhizobium loti
300
-
 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate mutant E149Q, pH 5.0, 30°C Mesorhizobium loti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Mesorhizobium loti

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Mesorhizobium loti

General Information

General Information Comment Organism
physiological function contrary to wild-type, mlr6793-disruptant cells cannot grow on pyridoxine, 4-pyridoxic acid or 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate as sole carbon and nitrogen source Mesorhizobium loti