Literature summary for 1.1.99.42 extracted from

Mugo, A.N.; Kobayashi, J.; Mikami, B.; Yoshikane, Y.; Yagi, T.; Ohnishi, K.
Crystal structure of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase from Mesorhizobium loti (2015), Biochem. Biophys. Res. Commun., 456, 35-40 .

Search for more literature for 1.1.99.42

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mesorhizobium japonicum

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure determined by molecular replacement, to 1. 55 A resolution. Residues Ser116, His137 and Glu149 are connected by a hydrogen bonding network forming a catalytic triad	Mesorhizobium japonicum

Organism

Organism	UniProt	Comment	Textmining
Mesorhizobium japonicum	Q988C8	enzyme additionally catalyzes the reaction of EC 1.2.1.100	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-pyridoxate + NAD+	-	Mesorhizobium japonicum	5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+	-	?
additional information	enzyme catalyzes oxidation of the hemiacetal form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 3-hydroxy-2-methylpyridine-4,5-dicarboxylate with NAD+, reaction of EC 1.2.1.100, and reduction of an aldehyde form of 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate to 4-pyridoxic acid with NADH. The Ser-His-Glu catalytic triad facilitates the two-way reactions. Ser116 assists protonation of His137 to drive the reduction reaction. His137 acts as a catalytic base to abstract a proton during oxidation. Glu149 likely neutralizes the positive charge on His137 after the deprotonation of the substrate	Mesorhizobium japonicum	?	-	?

Synonyms

Synonyms	Comment	Organism
mlr6793	-	Mesorhizobium japonicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Mesorhizobium japonicum

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Mesorhizobium japonicum

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Release 2023.1 (January 2023)