BRENDA - Enzyme Database show
show all sequences of 1.1.99.41

Completion of the seven-step pathway from tabersonine to the anticancer drug precursor vindoline and its assembly in yeast

Qu, Y.; Easson, M.L.; Froese, J.; Simionescu, R.; Hudlicky, T.; De Luca, V.; Proc. Natl. Acad. Sci. USA 112, 6224-6229 (2015)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
16-methoxytabersonine + NADPH + H+
Catharanthus roseus
-
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
?
tabersonine + NADPH + H+
Catharanthus roseus
-
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
A0A161CAI1
cultivar Little Delicata
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
epidermis
-
Catharanthus roseus
-
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16-hydroxytabersonine + NADPH + H+
-
743687
Catharanthus roseus
?
-
-
-
?
16-methoxytabersonine + NADPH + H+
-
743687
Catharanthus roseus
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
-
?
2,3-dihydrotabersonine + NADPH + H+
-
743687
Catharanthus roseus
?
-
-
-
?
tabersonine + NADPH + H+
-
743687
Catharanthus roseus
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
16-methoxytabersonine + NADPH + H+
Catharanthus roseus
-
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
?
tabersonine + NADPH + H+
Catharanthus roseus
-
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
epidermis
-
Catharanthus roseus
-
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
16-hydroxytabersonine + NADPH + H+
-
743687
Catharanthus roseus
?
-
-
-
?
16-methoxytabersonine + NADPH + H+
-
743687
Catharanthus roseus
3-hydroxy-16-methoxy-2,3-dihydrotabersonine
-
-
-
?
2,3-dihydrotabersonine + NADPH + H+
-
743687
Catharanthus roseus
?
-
-
-
?
tabersonine + NADPH + H+
-
743687
Catharanthus roseus
2,3-dihydro-3-hydroxytabersonine + NADP+
-
-
-
?
Other publictions for EC 1.1.99.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743560
Edge
A tabersonine 3-reductase Cat ...
Catharanthus roseus
Planta
247
155-169
2018
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743687
Qu
Completion of the seven-step ...
Catharanthus roseus
Proc. Natl. Acad. Sci. USA
112
6224-6229
2015
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