Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Niallia circulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-scyllo-inosamine | substrate inhibition | Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
2-deoxy-scyllo-inosamine | pH 8.0, 28°C | Niallia circulans | |
0.46 | - |
S-adenosyl-L-methionine | pH 8.0, 28°C | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | Q8G907 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine | enzyme catalyzes the oxidation of 2-deoxy-scyllo-inosamine under strictly anaerobic conditions along with consumption of an equimolar amount of S-adenosyl-L-methionine to produce 5-deoxyadenosine, methionine, and 3-amino-2,3-dideoxy-scylloinososeReaction follows the ordered Bi Ter mechanism and S-adenosyl-L-methionine is the first substrate and 2-deoxy-scyllo-inosamine is the second. The hydrogen atom at C-3 of 2-deoxy-scyllo-inosamine is directly transferred to 5-deoxyadenosine to give the radical intermediate of 2-deoxy-scyllo-inosamine in a reversible hydrogen abstraction step | Niallia circulans | 3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine | - |
? | |
2-deoxystreptamine + S-adenosyl-L-methionine | 14.4% of the rate with 2-deoxy-scyllo-inosamine | Niallia circulans | ? + 5'-deoxyadenosine + L-methionine | - |
? | |
additional information | no substrates: 1D-chiro-inositol, 1L-chiro-inositol, muco-inositol, allo-inositol, D-glucose, D-glucosamine, D-xylose | Niallia circulans | ? | - |
? | |
myo-inositol + S-adenosyl-L-methionine | 0.9% of the rate with 2-deoxy-scyllo-inosamine | Niallia circulans | ? + 5'-deoxyadenosine + L-methionine | - |
? | |
scyllo-inositol + S-adenosyl-L-methionine | 3.3% of the rate with 2-deoxy-scyllo-inosamine | Niallia circulans | ? + 5'-deoxyadenosine + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BtrN | - |
Niallia circulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
S-adenosyl-L-methionine | pH 8.0, 28°C | Niallia circulans | |
0.033 | - |
2-deoxy-scyllo-inosamine | pH 8.0, 28°C | Niallia circulans |
General Information | Comment | Organism |
---|---|---|
physiological function | gene disruption causes the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine and 2-deoxystreptamine. In vitro assay of the overexpressed enzyme reveals that the enzyme catalyzes the oxidation of 2-deoxy-scyllo-inosamine under strictly anaerobic conditions along with consumption of an equimolar amount of S-adenosyl-L-methionine to produce 5-deoxyadenosine, methionine, and 3-amino-2,3-dideoxy-scyllo-inosose | Niallia circulans |