Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the low coenzyme NAD-dependent activity of MDH with C1-C4 primary alcohols is strongly stimulated by Bacillus methanolicus protein ACT, in presence of NAD(H) cofactor and Mg2+-ions. In the deduced ACT amino acid sequence, the highly conserved amino acid sequence motif typical of Nudix hydrolase proteins is present. MDH activation by ACT involves hydrolytic removal of the nicotinamide mononucleotide NMN(H) moiety of the NAD(H) cofactor of MDH, changing its Ping-Pong type of reaction mechanism into a ternary complex reaction mechanism | Bacillus methanolicus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus methanolicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bacillus methanolicus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | one zinc and two magnesium ions per subunit. Mg2+ is essential for binding of the NAD(H) cofactor | Bacillus methanolicus | |
Zn2+ | one zinc and two magnesium ions per subunit | Bacillus methanolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
methanol + acceptor = formaldehyde + reduced acceptor | the low coenzyme NAD-dependent activity of MDH with C1-C4 primary alcohols is strongly stimulated by Bacillus methanolicus protein ACT, in presence of NAD(H) cofactor and Mg2+-ions. MDH activation by ACT involves hydrolytic removal of the nicotinamide mononucleotide NMN(H) moiety of the NAD(H) cofactor of MDH, changing its Ping-Pong type of reaction mechanism into a ternary complex reaction mechanism | Bacillus methanolicus |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Bacillus methanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | Mg2+ is essential for binding of the NAD(H) cofactor | Bacillus methanolicus | |
NADH | Mg2+ is essential for binding of the NAD(H) cofactor | Bacillus methanolicus |