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Literature summary for 1.1.99.36 extracted from

  • Van Ophem, P.W.; Van Beeumen, J.; Duine, J.A.
    Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica (1993), Eur. J. Biochem., 212, 819-826.
    View publication on PubMed
Search for more literature for 1.1.99.36

Activating Compound

Activating Compound Comment Organism Structure
additional information NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity Amycolatopsis methanolica
additional information no activation by phosphate and ammonium chloride. NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity Amycolatopsis methanolica

Inhibitors

Inhibitors Comment Organism Structure
1-butanol substrate inhibition Amycolatopsis methanolica
1-propanol substrate inhibition Amycolatopsis methanolica
2-butanol substrate inhibition Amycolatopsis methanolica
2-propanol substrate inhibition Amycolatopsis methanolica
acetaldehyde 1 mM, 40% inhibition; 40% inhibition at 1 mM, 60% at 2 mM, and 95% at 10 mM Amycolatopsis methanolica
ADP 10% inhibition at 1 mM, 50% at 10 mM Amycolatopsis methanolica
AMP 10% inhibition at 1 mM, 50% at 10 mM Amycolatopsis methanolica
ATP 10% inhibition at 1 mM, 50% at 10 mM Amycolatopsis methanolica
Cu2+ 1 mM, 90% inhibition; 90% inhibition at 1 mM Amycolatopsis methanolica
ethanol substrate inhibition Amycolatopsis methanolica
Fe2+ 1 mM, 90% inhibition; 90% inhibition at 1 mM Amycolatopsis methanolica
Hg2+ 100% inhibition at 1 mM Amycolatopsis methanolica
KCN 2 mM, 90% inhibition; 90% inhibition at 2 mM Amycolatopsis methanolica
additional information NAD(H) and NADP(H) do neither inhibit nor stimulate NDMA-ADH activity; NAD(H) and NADP(H) neither inhibit nor stimulate NDMA-ADH activity Amycolatopsis methanolica
NaN3 25% inhibition at 2 mM; 2 mM, 25% inhibition Amycolatopsis methanolica
Ni2+ 15% inhibition at 1 mM Amycolatopsis methanolica
Zn2+ 1 mM, 40% inhibition; 40% inhibition at 1 mM Amycolatopsis methanolica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Amycolatopsis methanolica
0.0025
-
 1-butanol pH 7.0, 45°C Amycolatopsis methanolica
0.0039
-
 1-propanol pH 7.0, 45°C Amycolatopsis methanolica
0.082
-
 ethanol pH 7.0, 45°C Amycolatopsis methanolica
10.1
-
 2-butanol pH 7.0, 45°C Amycolatopsis methanolica
13.4
-
 2-propanol pH 7.0, 45°C Amycolatopsis methanolica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 3 * 39000, SDS-PAGE Amycolatopsis methanolica
110000
-
 PAGE Amycolatopsis methanolica
110000
-
 native PAGE Amycolatopsis methanolica
120000
-
 gel filtration Amycolatopsis methanolica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + N,N-dimethyl-4-nitrosoaniline Amycolatopsis methanolica
-
 acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
ethanol + N,N-dimethyl-4-nitrosoaniline Amycolatopsis methanolica NCIB 11946
-
 acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
methanol + N,N-dimethyl-4-nitrosoaniline Amycolatopsis methanolica
-
 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
methanol + N,N-dimethyl-4-nitrosoaniline Amycolatopsis methanolica NCIB 11946
-
 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?

Organism

Organism UniProt Comment Textmining
Amycolatopsis methanolica
-
-
-
Amycolatopsis methanolica P80175
-
-
Amycolatopsis methanolica NCIB 11946
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 163fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, anion exchange and hydroxyapatite chromatography, followed by gel filtration Amycolatopsis methanolica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
 substrate methanol, pH 7.0, 45°C, extracts of cells grown on ethanol Amycolatopsis methanolica
0.004
-
 substrate ethanol, pH 7.0, 45°C, extracts of cells grown on ethanol Amycolatopsis methanolica
0.005
-
 substrate methanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol Amycolatopsis methanolica
0.018
-
 substrate ethanol, pH 7.0, 45°C, extracts of cells grown in fed-batch culture on methanol Amycolatopsis methanolica
3.1
-
 45°C, pH 7.0, purified enzyme Amycolatopsis methanolica
3.1
-
 purified enzyme, pH 7.0, 45°C Amycolatopsis methanolica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica butanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
1-butanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica butanal + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
1-butanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica NCIB 11946 butanal + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
1-hexanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica hexanal + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
1-propanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica propanaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
2-butanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica butanone + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
2-propanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica acetone + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
acetate + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica ? + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
ethanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
ethanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica NCIB 11946 acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
methanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
methanol + N,N-dimethyl-4-nitrosoaniline best substrate Amycolatopsis methanolica formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
methanol + N,N-dimethyl-4-nitrosoaniline
-
 Amycolatopsis methanolica NCIB 11946 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
methanol + N,N-dimethyl-4-nitrosoaniline best substrate Amycolatopsis methanolica NCIB 11946 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 ?
additional information enzyme shows formaldehyde dismutase activity producing methylformate when incubated with formaldehyde and methanol Amycolatopsis methanolica ?
-
 ?
additional information no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c Amycolatopsis methanolica ?
-
 ?
additional information no activity with electron acceptors NAD+, NADP+, 3-acetylpyridine adenine dinucleotide, thionicotinamide adenine dinucleotide, phenazine methosulfate, Wurster's Blue, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliurn bromide, or cytochrome c Amycolatopsis methanolica NCIB 11946 ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 39000, SDS-PAGE Amycolatopsis methanolica

Synonyms

Synonyms Comment Organism
4-nitroso-N, N-dimethylaniline-dependent alcohol dehydrogenase
-
 Amycolatopsis methanolica
NDMA-ADH
-
 Amycolatopsis methanolica
NDMA-dependent alcohol dehydrogenase
-
 Amycolatopsis methanolica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Amycolatopsis methanolica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Amycolatopsis methanolica

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADPH Amycolatopsis methanolica
NADH 1 mol NADH per mol of enzyme trimer Amycolatopsis methanolica
NADH tightly bound, but NDMA-dependent alcohol dehydrogenase is unable to use NAD(P)(H) as cofactors Amycolatopsis methanolica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.65
-
 1-butanol pH 7.0, 45°C Amycolatopsis methanolica
4
-
 1-propanol pH 7.0, 45°C Amycolatopsis methanolica
4.4
-
 2-butanol pH 7.0, 45°C Amycolatopsis methanolica
14.5
-
 2-propanol pH 7.0, 45°C Amycolatopsis methanolica
33
-
 ethanol pH 7.0, 45°C Amycolatopsis methanolica