Any feedback?
Literature summary for 1.1.99.35 extracted from
- Designing a highly active soluble PQQ-glucose dehydrogenase for efficient glucose biosensors and biofuel cells (2010), Biochem. Biophys. Res. Commun., 402, 750-754.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Acinetobacter calcoaceticus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N428C | the catalytic efficiency is increased by 47% compared to the wild type enzyme, the mutant shows increased affinity for pyrroloquinoline quinone and is twice more active toward D-glucose and more selective toward maltose than the wild type | Acinetobacter calcoaceticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 43 | - |
D-glucose | wild type enzyme, at pH 7.0 and 37°C | Acinetobacter calcoaceticus |  |
| 64 | - |
D-glucose | mutant enzyme N428C, at pH 7.0 and 37°C | Acinetobacter calcoaceticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | - |
D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | P13650 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + oxidized 2,6-dichlorophenolindophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PQQ-GDH | - |
Acinetobacter calcoaceticus |
| soluble PQQ-glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
