Literature summary for 1.1.99.35 extracted from

Sode, K.; Igarashi, S.; Morimoto, A.; Yoshida, H.
Construction of engineered water-soluble PQQ glucose dehydrogenase with improved substrate specificity (2002), Biocatal. Biotransform., 20, 405-412.

No PubMed abstract available

Search for more literature for 1.1.99.35

Protein Variants

Protein Variants	Comment	Organism
D448N	substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 72%, D-allose 39%, D-galactose 14%, D-maltose 36%, D-lactose 48%, respectively	Acinetobacter calcoaceticus
D456N	substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 78%, D-allose 43%, D-galactose 16%, D-maltose 41%, D-lactose 59%, respectively	Acinetobacter calcoaceticus
DD457N	substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 80%, D-allose 46%, D-galactose 11%, D-maltose 32%, D-lactose 43%, respectively	Acinetobacter calcoaceticus
N452T	substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 59%, D-allose 33%, D-galactose 5%, D-maltose 30%, D-lactose 31%, respectively	Acinetobacter calcoaceticus
N462H	substrate specificity: D-glucose 100% activity, 3-O-methyl-D-glucose 53%, D-allose 32%, D-galactose 2%, D-maltose 25%, D-lactose 31%, respectively	Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7	-	D-galactose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
3.7	-	D-galactose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
5.3	-	D-galactose	wild-type, pH 7.0	Acinetobacter calcoaceticus
12.3	-	D-glucose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
12.5	-	D-glucose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
16	-	D-maltose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
18	-	D-lactose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
18.9	-	D-lactose	wild-type, pH 7.0	Acinetobacter calcoaceticus
25	-	D-glucose	wild-type, pH 7.0	Acinetobacter calcoaceticus
26	-	D-maltose	wild-type, pH 7.0	Acinetobacter calcoaceticus
27.6	-	3-O-methyl-D-glucose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
28.7	-	3-O-methyl-D-glucose	wild-type, pH 7.0	Acinetobacter calcoaceticus
28.8	-	3-O-methyl-D-glucose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
32.5	-	D-allose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
33.6	-	D-lactose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
35.5	-	D-allose	wild-type, pH 7.0	Acinetobacter calcoaceticus
38.7	-	D-allose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
46.5	-	D-maltose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter calcoaceticus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol	81% of the activity with D-glucose	Acinetobacter calcoaceticus	3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
D-allose + oxidized 2,6-dichlorophenolindophenol	47% of the activity with D-glucose	Acinetobacter calcoaceticus	D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
D-galactose + oxidized 2,6-dichlorophenolindophenol	11% of the activity with D-glucose	Acinetobacter calcoaceticus	D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
D-glucose + oxidized 2,6-dichlorophenolindophenol	-	Acinetobacter calcoaceticus	D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
lactose + oxidized 2,6-dichlorophenolindophenol	61% of the activity with D-glucose	Acinetobacter calcoaceticus	lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
maltose + oxidized 2,6-dichlorophenolindophenol	61% of the activity with D-glucose	Acinetobacter calcoaceticus	maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?

Synonyms

Synonyms	Comment	Organism
PQQ glucose dehydrogenase	-	Acinetobacter calcoaceticus
PQQGDH-B	-	Acinetobacter calcoaceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
69	-	D-galactose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
72	-	D-galactose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
232	-	D-galactose	wild-type, pH 7.0	Acinetobacter calcoaceticus
574	-	D-lactose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
588	-	D-maltose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
949	-	D-allose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
1002	-	D-maltose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
1035	-	D-allose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
1038	-	D-lactose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
1064	-	3-O-methyl-D-glucose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
1253	-	3-O-methyl-D-glucose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
1399	-	D-glucose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
1659	-	D-lactose	wild-type, pH 7.0	Acinetobacter calcoaceticus
1791	-	D-glucose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
1930	-	D-maltose	wild-type, pH 7.0	Acinetobacter calcoaceticus
2509	-	D-allose	wild-type, pH 7.0	Acinetobacter calcoaceticus
3011	-	3-O-methyl-D-glucose	wild-type, pH 7.0	Acinetobacter calcoaceticus
3860	-	D-glucose	wild-type, pH 7.0	Acinetobacter calcoaceticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
22	-	D-maltose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
23	-	D-galactose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
25	-	D-allose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
26	-	D-galactose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
31	-	D-lactose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
32	-	D-allose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
32	-	D-lactose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
37	-	D-maltose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
37	-	3-O-methyl-D-glucose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
44	-	D-galactose	wild-type, pH 7.0	Acinetobacter calcoaceticus
45	-	3-O-methyl-D-glucose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
71	-	D-allose	wild-type, pH 7.0	Acinetobacter calcoaceticus
74	-	D-maltose	wild-type, pH 7.0	Acinetobacter calcoaceticus
88	-	D-lactose	wild-type, pH 7.0	Acinetobacter calcoaceticus
105	-	3-O-methyl-D-glucose	wild-type, pH 7.0	Acinetobacter calcoaceticus
114	-	D-glucose	mutant N462H, pH 7.0	Acinetobacter calcoaceticus
143	-	D-glucose	mutant N452T, pH 7.0	Acinetobacter calcoaceticus
154	-	D-glucose	wild-type, pH 7.0	Acinetobacter calcoaceticus

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Release 2023.1 (January 2023)