Literature summary for 1.1.99.32 extracted from

Gao, L.; Du, G.; Zhou, J.; Chen, J.; Liu, J.
Characterization of a group of pyrroloquinoline quinone-dependent dehydrogenases that are involved in the conversion of L-sorbose to 2-keto-L-gulonic acid in Ketogulonicigenium vulgare WSH-001 (2013), Biotechnol. Prog., 29, 1398-1404.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Ketogulonicigenium vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-sorbose + 2,6-dichlorophenolindophenol	Ketogulonicigenium vulgare	-	L-sorbosone + reduced 2,6-dichlorophenolindophenol	-	?
L-sorbose + 2,6-dichlorophenolindophenol	Ketogulonicigenium vulgare WSH-001	-	L-sorbosone + reduced 2,6-dichlorophenolindophenol	-	?

Organism

Organism	UniProt	Comment	Textmining
Ketogulonicigenium vulgare	-	-	-
Ketogulonicigenium vulgare WSH-001	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography, and Superdex 200 gel filtration	Ketogulonicigenium vulgare

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-sorbose + 2,6-dichlorophenolindophenol	-	Ketogulonicigenium vulgare	L-sorbosone + reduced 2,6-dichlorophenolindophenol	-	?
L-sorbose + 2,6-dichlorophenolindophenol	-	Ketogulonicigenium vulgare WSH-001	L-sorbosone + reduced 2,6-dichlorophenolindophenol	-	?

Synonyms

Synonyms	Comment	Organism
PQQ-dependent L-sorbose dehydrogenase	-	Ketogulonicigenium vulgare
SDH	-	Ketogulonicigenium vulgare

Cofactor

Cofactor	Comment	Organism	Structure
pyrroloquinoline quinone	-	Ketogulonicigenium vulgare

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Release 2023.1 (January 2023)