BRENDA - Enzyme Database
show all sequences of 1.1.99.30

Additional experiences with Proteus vulgaris and its hydroxycarboxylate-oxidoreductase for the preparation of (2R)- or (2S)-hydroxycarboxylates

Krauss, F.; Guenther, H.; Skopan, H.; Simon, H.; DECHEMA Biotechnol. Conf. 1, 313-318 (1988)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
tungstate
complete inhibition in vivo
Proteus vulgaris
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron-sulfur cluster
-
Proteus vulgaris
molybdate
strong induction
Proteus vulgaris
Molybdenum
4-12 atoms per enzyme molecule
Proteus vulgaris
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
750000
-
-
Proteus vulgaris
Organism
Organism
UniProt
Commentary
Textmining
Proteus vulgaris
-
molybdate-inducible
-
Purification (Commentary)
Purification (Commentary)
Organism
60fold
Proteus vulgaris
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
810
-
crude cell extract
Proteus vulgaris
Storage Stability
Storage Stability
Organism
-16°C, N2-atmosphere, wet packed cells, 450 days, more than 90% remaining activity
Proteus vulgaris
room temperature, freeze dried cells, 200 days, more than 80% remaining activity
Proteus vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2R)-hydroxy-4-methylpentanoate + 2-oxo-butyrate
-
655314
Proteus vulgaris
2-oxo-4-methylpentanoate + (2R)-hydroxybutyrate
-
-
-
r
2-hydroxyglutarate + oxidized acceptor
-
655314
Proteus vulgaris
2-oxoglutarate + reduced acceptor
-
-
-
r
2-oxo-3,5-dienoates + H2
-
655314
Proteus vulgaris
?
-
-
-
r
2-oxo-3-(3,4-dihydroxyphenyl)-propionate + H2
-
655314
Proteus vulgaris
rosmarinic acid
-
-
-
r
2-oxo-3-enoates + H2
-
655314
Proteus vulgaris
?
-
-
-
r
lactate + oxidized acceptor
-
655314
Proteus vulgaris
pyruvate + reduced acceptor
-
-
-
r
mandelate + oxidized acceptor
-
655314
Proteus vulgaris
oxo(phenyl)acetic acid + reduced acceptor
-
-
-
r
pantolactone + oxidized acceptor
-
655314
Proteus vulgaris
dehydropantolactone + reduced acceptor
-
-
-
r
phenylpyruvate + H2
-
655314
Proteus vulgaris
(2R)-phenyllactate
-
-
-
r
phenylpyruvate + reduced benzyl viologen
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized benzyl viologen
-
-
-
r
phenylpyruvate + reduced carbamoylmethyl viologen
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized carbamoylmethyl viologen
-
-
-
r
phenylpyruvate + reduced cobalt sepulchrate
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized cobalt sepulchrate
-
-
-
r
phenylpyruvate + reduced indigocarmine
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized indigocarmine
-
-
-
r
phenylpyruvate + reduced kresyl violet
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized kresyl violet
-
-
-
r
phenylpyruvate + reduced meldolablue
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized meldolablue
-
-
-
r
phenylpyruvate + reduced methylene blue
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized methylene blue
-
-
-
r
phenylpyruvate + reduced phenosafranine
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized phenosafranine
-
-
-
r
phenylpyruvate + reduced safranine TH
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized safranine TH
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
HVOR
-
Proteus vulgaris
hydroxycarboxlate-oxidoreductase
-
Proteus vulgaris
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Proteus vulgaris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Proteus vulgaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
tungstate
complete inhibition in vivo
Proteus vulgaris
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron-sulfur cluster
-
Proteus vulgaris
molybdate
strong induction
Proteus vulgaris
Molybdenum
4-12 atoms per enzyme molecule
Proteus vulgaris
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
750000
-
-
Proteus vulgaris
Purification (Commentary) (protein specific)
Commentary
Organism
60fold
Proteus vulgaris
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
810
-
crude cell extract
Proteus vulgaris
Storage Stability (protein specific)
Storage Stability
Organism
-16°C, N2-atmosphere, wet packed cells, 450 days, more than 90% remaining activity
Proteus vulgaris
room temperature, freeze dried cells, 200 days, more than 80% remaining activity
Proteus vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2R)-hydroxy-4-methylpentanoate + 2-oxo-butyrate
-
655314
Proteus vulgaris
2-oxo-4-methylpentanoate + (2R)-hydroxybutyrate
-
-
-
r
2-hydroxyglutarate + oxidized acceptor
-
655314
Proteus vulgaris
2-oxoglutarate + reduced acceptor
-
-
-
r
2-oxo-3,5-dienoates + H2
-
655314
Proteus vulgaris
?
-
-
-
r
2-oxo-3-(3,4-dihydroxyphenyl)-propionate + H2
-
655314
Proteus vulgaris
rosmarinic acid
-
-
-
r
2-oxo-3-enoates + H2
-
655314
Proteus vulgaris
?
-
-
-
r
lactate + oxidized acceptor
-
655314
Proteus vulgaris
pyruvate + reduced acceptor
-
-
-
r
mandelate + oxidized acceptor
-
655314
Proteus vulgaris
oxo(phenyl)acetic acid + reduced acceptor
-
-
-
r
pantolactone + oxidized acceptor
-
655314
Proteus vulgaris
dehydropantolactone + reduced acceptor
-
-
-
r
phenylpyruvate + H2
-
655314
Proteus vulgaris
(2R)-phenyllactate
-
-
-
r
phenylpyruvate + reduced benzyl viologen
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized benzyl viologen
-
-
-
r
phenylpyruvate + reduced carbamoylmethyl viologen
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized carbamoylmethyl viologen
-
-
-
r
phenylpyruvate + reduced cobalt sepulchrate
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized cobalt sepulchrate
-
-
-
r
phenylpyruvate + reduced indigocarmine
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized indigocarmine
-
-
-
r
phenylpyruvate + reduced kresyl violet
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized kresyl violet
-
-
-
r
phenylpyruvate + reduced meldolablue
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized meldolablue
-
-
-
r
phenylpyruvate + reduced methylene blue
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized methylene blue
-
-
-
r
phenylpyruvate + reduced phenosafranine
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized phenosafranine
-
-
-
r
phenylpyruvate + reduced safranine TH
-
655314
Proteus vulgaris
(2R)-phenyllactate + oxidized safranine TH
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Proteus vulgaris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Proteus vulgaris
Other publictions for EC 1.1.99.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287842
Hekmat
-
Production of pyruvate from (R ...
Proteus vulgaris
Enzyme Microb. Technol.
24
471-479
1999
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655353
Andersson
-
Microbial production of D-(S)- ...
Proteus vulgaris
Enzyme Microb. Technol.
22
170-178
1998
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
2
1
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287841
Trautwein
The (2R)-hydroxycarboxylate-vi ...
Proteus vulgaris
Eur. J. Biochem.
222
1025-1032
1994
-
-
-
-
-
-
2
-
1
-
2
-
-
3
-
-
1
-
-
1
1
-
7
2
1
2
-
-
-
1
-
-
2
-
1
-
-
-
-
2
-
-
-
-
2
-
-
1
-
2
-
-
-
-
1
-
1
1
-
7
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
654958
Schinschel
Proteus mirabilis dehydrogenat ...
Proteus mirabilis
Bioorg. Med. Chem.
2
483-491
1994
-
-
-
-
-
-
-
16
-
-
-
-
-
1
-
-
-
1
-
-
-
-
42
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
42
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
655713
Simon
-
Properties and mechanistic asp ...
Proteus vulgaris
Indian J. Chem. B
32
170-175
1993
-
1
-
-
-
-
-
-
1
1
3
-
-
1
-
-
-
1
-
-
1
2
5
1
6
-
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
1
3
-
-
-
-
-
-
-
1
2
5
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
655314
Krauss
-
Additional experiences with Pr ...
Proteus vulgaris
DECHEMA Biotechnol. Conf.
1
313-318
1988
-
-
-
-
-
-
1
-
-
3
1
-
-
1
-
-
1
-
-
-
1
2
18
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
1
-
-
-
-
1
-
-
1
2
18
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644550
Neumann
-
On a non-pyridine nucleotide-d ...
Proteus mirabilis, Proteus sp., Proteus vulgaris
FEBS Lett.
167
29-32
1985
-
2
-
-
-
-
4
-
2
1
-
-
-
3
-
-
2
-
-
2
1
-
47
-
-
1
-
2
-
-
-
-
2
-
-
-
-
2
-
2
-
-
-
-
4
-
-
2
1
-
-
-
-
-
2
-
2
1
-
47
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-