BRENDA - Enzyme Database
show all sequences of 1.1.99.30

The (2R)-hydroxycarboxylate-viologen-oxidoreductase from Proteus vulgaris is a molybdenum-containing iron-sulphur protein

Trautwein, T.; Krauss, F.; Lottspeich, F.; Simon, H.; Eur. J. Biochem. 222, 1025-1032 (1994)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CN-
-
Proteus vulgaris
O2
oxygen-sensitive
Proteus vulgaris
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Proteus vulgaris
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
8 * 80000, SDS-PAGE
Proteus vulgaris
600000
-
gel filtration
Proteus vulgaris
Organism
Organism
UniProt
Commentary
Textmining
Proteus vulgaris
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
anaerobic conditions
Proteus vulgaris
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
anaerobic growth conditions
Proteus vulgaris
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1800
-
purified holoenzyme
Proteus vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a (2R)-hydroxy-carboxylate + acceptor
-
287841
Proteus vulgaris
a 2-oxo-carboxylate + reduced acceptor
-
-
-
?
D-gluconate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
D-glucose 6-phosphate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
D-lactate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
pyruvate + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
N-acetylneuraminate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
phenylpyruvate + reduced 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
phenyllactate + oxidized 1,1'-carbamoyl methyl viologen
-
-
-
r
phenylpyruvate + reduced benzyl viologen
anaerobic conditions
287841
Proteus vulgaris
phenyllactate + oxidized benzyl viologen
-
-
-
r
Subunits
Subunits
Commentary
Organism
More
subunit structure
Proteus vulgaris
octamer
8 * 80000, SDS-PAGE
Proteus vulgaris
Synonyms
Synonyms
Commentary
Organism
HVOR
-
Proteus vulgaris
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
dehydrogenase assay at
Proteus vulgaris
37
-
reductase assay at
Proteus vulgaris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Proteus vulgaris
Cofactor
Cofactor
Commentary
Organism
Structure
molybdenum cofactor
mononucleotide, 1 enzyme molecule contains 1 molybdenum atom
Proteus vulgaris
additional information
enzyme contains iron sulfur clusters, with four iron and 4 sulfur atoms per enzyme molecule, enzyme belongs to the group of molybdoenzymes which possess no further prosthetic group than the iron sulfur clusters
Proteus vulgaris
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Proteus vulgaris
isoelectric focusing
-
4.9
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
molybdenum cofactor
mononucleotide, 1 enzyme molecule contains 1 molybdenum atom
Proteus vulgaris
additional information
enzyme contains iron sulfur clusters, with four iron and 4 sulfur atoms per enzyme molecule, enzyme belongs to the group of molybdoenzymes which possess no further prosthetic group than the iron sulfur clusters
Proteus vulgaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CN-
-
Proteus vulgaris
O2
oxygen-sensitive
Proteus vulgaris
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Proteus vulgaris
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
80000
-
8 * 80000, SDS-PAGE
Proteus vulgaris
600000
-
gel filtration
Proteus vulgaris
Purification (Commentary) (protein specific)
Commentary
Organism
anaerobic conditions
Proteus vulgaris
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
anaerobic growth conditions
Proteus vulgaris
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1800
-
purified holoenzyme
Proteus vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a (2R)-hydroxy-carboxylate + acceptor
-
287841
Proteus vulgaris
a 2-oxo-carboxylate + reduced acceptor
-
-
-
?
D-gluconate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
D-glucose 6-phosphate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
D-lactate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
pyruvate + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
N-acetylneuraminate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
phenylpyruvate + reduced 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
phenyllactate + oxidized 1,1'-carbamoyl methyl viologen
-
-
-
r
phenylpyruvate + reduced benzyl viologen
anaerobic conditions
287841
Proteus vulgaris
phenyllactate + oxidized benzyl viologen
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
More
subunit structure
Proteus vulgaris
octamer
8 * 80000, SDS-PAGE
Proteus vulgaris
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
dehydrogenase assay at
Proteus vulgaris
37
-
reductase assay at
Proteus vulgaris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Proteus vulgaris
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Proteus vulgaris
isoelectric focusing
-
4.9
Other publictions for EC 1.1.99.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287842
Hekmat
-
Production of pyruvate from (R ...
Proteus vulgaris
Enzyme Microb. Technol.
24
471-479
1999
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655353
Andersson
-
Microbial production of D-(S)- ...
Proteus vulgaris
Enzyme Microb. Technol.
22
170-178
1998
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
2
1
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287841
Trautwein
The (2R)-hydroxycarboxylate-vi ...
Proteus vulgaris
Eur. J. Biochem.
222
1025-1032
1994
-
-
-
-
-
-
2
-
1
-
2
-
-
3
-
-
1
-
-
1
1
-
7
2
1
2
-
-
-
1
-
-
2
-
1
-
-
-
-
2
-
-
-
-
2
-
-
1
-
2
-
-
-
-
1
-
1
1
-
7
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
654958
Schinschel
Proteus mirabilis dehydrogenat ...
Proteus mirabilis
Bioorg. Med. Chem.
2
483-491
1994
-
-
-
-
-
-
-
16
-
-
-
-
-
1
-
-
-
1
-
-
-
-
42
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
42
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
655713
Simon
-
Properties and mechanistic asp ...
Proteus vulgaris
Indian J. Chem. B
32
170-175
1993
-
1
-
-
-
-
-
-
1
1
3
-
-
1
-
-
-
1
-
-
1
2
5
1
6
-
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
1
3
-
-
-
-
-
-
-
1
2
5
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
655314
Krauss
-
Additional experiences with Pr ...
Proteus vulgaris
DECHEMA Biotechnol. Conf.
1
313-318
1988
-
-
-
-
-
-
1
-
-
3
1
-
-
1
-
-
1
-
-
-
1
2
18
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
1
-
-
-
-
1
-
-
1
2
18
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644550
Neumann
-
On a non-pyridine nucleotide-d ...
Proteus mirabilis, Proteus sp., Proteus vulgaris
FEBS Lett.
167
29-32
1985
-
2
-
-
-
-
4
-
2
1
-
-
-
3
-
-
2
-
-
2
1
-
47
-
-
1
-
2
-
-
-
-
2
-
-
-
-
2
-
2
-
-
-
-
4
-
-
2
1
-
-
-
-
-
2
-
2
1
-
47
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-