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Literature summary for 1.1.99.29 extracted from

  • Tan, T.; Spadiut, O.; Wongnate, T.; Sucharitakul, J.; Krondorfer, I.; Sygmund, C.; Haltrich, D.; Chaiyen, P.; Peterbauer, C.; Divne, C.
    The 1.6 A crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate specificity and reveals a flavin intermediate (2013), PLoS ONE, 8, e53567.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Leucoagaricus meleagris

Crystallization (Commentary)

Crystallization (Comment) Organism
microseeding, using 50 mM KH2PO4, 5% (v/v) 2-methyl-2,4-pentanediol, and 20% (w/v) polyethylene glycol 8000 Leucoagaricus meleagris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a pyranoside + acceptor Leucoagaricus meleagris
-
 a 3-dehydropyranoside + reduced acceptor 3-dehydropyranoside or 3,4-didehydropyranoside ?
D-glucose + acceptor Leucoagaricus meleagris the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack 2-dehydro-D-glucose + reduced acceptor
-
 ?
additional information Leucoagaricus meleagris O2 is a poor electron acceptor ?
-
 ?
pyranose + acceptor Leucoagaricus meleagris
-
 2-dehydropyranose + reduced acceptor 2-dehydropyranose or 1-dehydropyranose or 3-dehydropyranose or 2,3-didehydropyranose ?

Organism

Organism UniProt Comment Textmining
Leucoagaricus meleagris Q3L245
-
-

Purification (Commentary)

Purification (Comment) Organism
hydrophobic interaction chromatography and anion exchange chromatography Leucoagaricus meleagris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-fluoro-3-deoxy-D-glucose + acceptor
-
 Leucoagaricus meleagris ? + reduced acceptor
-
 ?
a pyranoside + acceptor
-
 Leucoagaricus meleagris a 3-dehydropyranoside + reduced acceptor 3-dehydropyranoside or 3,4-didehydropyranoside ?
D-glucose + acceptor the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack Leucoagaricus meleagris 2-dehydro-D-glucose + reduced acceptor
-
 ?
additional information O2 is a poor electron acceptor Leucoagaricus meleagris ?
-
 ?
pyranose + acceptor
-
 Leucoagaricus meleagris 2-dehydropyranose + reduced acceptor 2-dehydropyranose or 1-dehydropyranose or 3-dehydropyranose or 2,3-didehydropyranose ?

Synonyms

Synonyms Comment Organism
PDH
-
 Leucoagaricus meleagris

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Leucoagaricus meleagris