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Literature summary for 1.1.99.29 extracted from

  • Tasca, F.; Timur, S.; Ludwig, R.; Haltrich, D.; Volc, J.; Antiochia, R.; Gorton, L.
    Amperometric biosensors for detection of sugars based on the electrical wiring of different pyranose oxidases and pyranose dehydrogenases with osmium redox polymer on graphite electrodes (2007), Electroanalysis, 19, 294-302.
No PubMed abstract available
Search for more literature for 1.1.99.29

Application

Application Comment Organism
biotechnology PDH could be a very interesting alternative to pyranose oxidase for applications in biotechnology or biofuel cells, electrical wiring of the enzyme bound to graphite rod electrodes is studied Agaricus xanthodermus
biotechnology PDH is an alternative to pyranose oxidase for applications in biotechnology or biofuel cells, electrical wiring of the enzyme bound to graphite rod electrodes is studied Leucoagaricus meleagris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 D-trehalose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
0.34
-
 D-maltose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
0.35
-
 sucrose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
0.44
-
 D-glucose at 0.1 M ethanolamine buffer at pH 9.4 Agaricus xanthodermus
0.51
-
 D-maltoheptaose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
0.62
-
 D-mannose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
0.64
-
 D-maltotriose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
0.64
-
 D-maltopentaose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
0.65
-
 D-glucose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
0.65
-
 2-deoxy-D-glucose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
0.74
-
 D-glucosamine 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
1.05
-
 D-cellobiose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
5.84
-
 D-xylose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
7.16
-
 D-galactose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
8.63
-
 L-arabinose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
10.84
-
 beta-lactose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
12.14
-
 2-deoxy-D-galactose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris
12.31
-
 L-fucose 0.1 M ethanolamine buffer at pH 9.4 Leucoagaricus meleagris

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Leucoagaricus meleagris
-
-
extracellular
-
 Agaricus xanthodermus
-
-

Organism

Organism UniProt Comment Textmining
Agaricus xanthodermus Q3L1D2
-
-
Leucoagaricus meleagris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography Leucoagaricus meleagris
using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography Agaricus xanthodermus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-galactose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
2-deoxy-D-glucose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
beta-lactose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-cellobiose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-galactose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-glucosamine + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-glucose + acceptor
-
 Leucoagaricus meleagris 3-dehydro-D-glucose + reduced acceptor
-
 ?
D-glucose + acceptor
-
 Agaricus xanthodermus 3-dehydro-D-glucose + reduced acceptor
-
 ?
D-maltoheptaose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-maltopentaose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-maltose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-maltotriose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-mannose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-trehalose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
D-xylose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
L-arabinose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
L-fucose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?
sucrose + acceptor
-
 Leucoagaricus meleagris ?
-
 ?

Synonyms

Synonyms Comment Organism
PDH AM
-
 Leucoagaricus meleagris
PDH AX
-
 Agaricus xanthodermus
pyranose dehydrogenase
-
 Leucoagaricus meleagris
pyranose dehydrogenase
-
 Agaricus xanthodermus
pyranose:acceptor oxidoreductase
-
 Leucoagaricus meleagris
pyranose:acceptor oxidoreductase
-
 Agaricus xanthodermus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM D-glucose, flow rate 1.0 mL/min Leucoagaricus meleagris
9
-
 in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 mL/min Agaricus xanthodermus

pH Range

pH Minimum pH Maximum Comment Organism
7 8 in PBS, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min Leucoagaricus meleagris
7 10.5 depending on the buffer, highest biosensor response in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min Leucoagaricus meleagris
8 10 in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 mL/min Agaricus xanthodermus
8 9 in Tris, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min Leucoagaricus meleagris