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Literature summary for 1.1.99.28 extracted from

  • Wiegert, T.; Sahm, H.; Sprenger, G.A.
    Expression of the Zymomonas mobilis gfo gene or NADP-containing glucose:fructose oxidoreductase (GFOR) in Escherichia coli. Formation of enzymatically active preGFOR but lack of processing into a stable periplasmic protein (1997), Eur. J. Biochem., 224, 107-112.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Zymomonas mobilis
NADP+ is required for the enzyme to fold into its native conformation and its absence from the Escherichia coli periplasm is responsible for failure to form a stable periplasmic protein Zymomonas mobilis

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Zymomonas mobilis
-
-

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + D-fructose
-
Zymomonas mobilis D-glucono-1,5-lactone + D-sorbitol
-
?

Cofactor

Cofactor Comment Organism Structure
NADP+ tightly bound as hydrogen carrier Zymomonas mobilis
NADPH tightly bound as hydrogen carrier Zymomonas mobilis