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Literature summary for 1.1.99.24 extracted from

  • Kardon, T.; Noel, G.; Vertommen, D.; Van Schaftingen, E.
    Identification of the gene encoding hydroxyacid-oxoacid transhydrogenase, an enzyme that metabolizes 4-hydroxybutyrate (2006), FEBS Lett., 580, 2347-2350.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect by EDTA and EGTA Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in HEK-293 cells as soluble protein Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline IC50: 0.75 mM Rattus norvegicus
cyanide IC50: 0.075 mM Rattus norvegicus
additional information no effect by EDTA and EGTA; no inhibition with EDTA, EGTA Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information no activity with L-isomer of 2-hydroxybutyrate, Km for succinate semialdehyde is below 0.01 mM, 30°C, pH 7.1 Rattus norvegicus
0.01
-
Succinic semialdehyde below, pH 7.1, 30°C Rattus norvegicus
0.06
-
4-hydroxybutyrate pH 7.1, 30°C Rattus norvegicus
0.06
-
4-hydroxybutyrate +/- 0.006 mM, 30°C, pH 7.1 Rattus norvegicus
0.067
-
4-hydroxybutyrate +/-0.012 mM, in overepressed HEK cells, in presence of 0.5 mM 2-oxoglutarate Homo sapiens
0.067
-
4-hydroxybutyrate pH 7.1, 30°C, recombinant enzyme Mus musculus
0.077
-
2-oxoglutarate +/-0.009 mM, in overexpressed HEK cells, in presence of 0.1 mM 4-hydroxybutyrate Homo sapiens
0.077
-
2-oxoglutarate pH 7.1, 30°C, recombinant enzyme Mus musculus
0.085
-
2-oxoglutarate pH 7.1, 30°C Rattus norvegicus
0.085
-
2-oxoglutarate +/-0.007 mM, 30°C, pH 7.1 Rattus norvegicus
4.5
-
D-2-hydroxyglutarate pH 7.1, 30°C Rattus norvegicus
4.5
-
D-2-hydroxybutyrate no activity with L-isomer, 30°C, pH 7.1 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information bi or trivalent, no stimulation with 0.01-0.05 mM Fe2+, Fe3+, Co2+, Ni2+, Mn2+, Zn2+, Mg2+ or Ca2+ Rattus norvegicus
additional information not affected by 0.5 mM Fe2+, Fe3+, Co2+, Ni2+, Mn2+, and Zn2+, or 1 mM Mg2+ and Ca2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000 55000 gel filtration Rattus norvegicus
45000 50000
-
Rattus norvegicus
50000
-
1 * 50000, about, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxybutyrate + 2-oxoglutarate Homo sapiens
-
D-2-hydroxyglutarate + succinate semialdehyde
-
?
4-hydroxybutyrate + 2-oxoglutarate Rattus norvegicus
-
D-2-hydroxyglutarate + succinate semialdehyde
-
?
4-hydroxybutyrate + 2-oxoglutarate Rattus norvegicus
-
succinic semialdehyde + D-2-hydroxyglutarate
-
r
4-hydroxybutyrate + 2-oxoglutarate Mus musculus recombinant enzyme in HEK-293 cells succinic semialdehyde + D-2-hydroxyglutarate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1300fold, partial from liver Rattus norvegicus
native enzyme from liver 1300fold by precipitation using PEG 6000, anion exchange and hydrophobic interaction chromatography, and gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain low enzyme activity Rattus norvegicus
-
heart low enzyme activity Rattus norvegicus
-
heart highest activity in liver and kidney and an intermediate activity in heart Rattus norvegicus
-
HEK-293 cell mouse homologue expressed in Homo sapiens
-
kidney
-
Rattus norvegicus
-
kidney highest activity in liver and kidney and an intermediate activity in heart Rattus norvegicus
-
liver
-
Rattus norvegicus
-
liver highest activity in liver and kidney and an intermediate activity in heart Rattus norvegicus
-
lung very low enzyme activity Rattus norvegicus
-
additional information tissue distribution, overview Rattus norvegicus
-
additional information no activity in brain and skeletal muscle Rattus norvegicus
-
skeletal muscle low enzyme activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0013
-
recombinant soluble enzyme in HEK-293 cells Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybutyrate + 2-oxoglutarate
-
Homo sapiens D-2-hydroxyglutarate + succinate semialdehyde
-
?
4-hydroxybutyrate + 2-oxoglutarate
-
Rattus norvegicus D-2-hydroxyglutarate + succinate semialdehyde
-
?
4-hydroxybutyrate + 2-oxoglutarate
-
Mus musculus succinic semialdehyde + D-2-hydroxyglutarate
-
?
4-hydroxybutyrate + 2-oxoglutarate
-
Rattus norvegicus succinic semialdehyde + D-2-hydroxyglutarate
-
r
4-hydroxybutyrate + 2-oxoglutarate recombinant enzyme in HEK-293 cells Mus musculus succinic semialdehyde + D-2-hydroxyglutarate
-
?
4-hydroxybutyrate + 2-oxoglutarate stereospecific reaction, no activity with L-2-hydroxyglutarate Rattus norvegicus succinic semialdehyde + D-2-hydroxyglutarate
-
r
D-2-hydroxybutyrate + 2-oxoglutarate
-
Rattus norvegicus ?
-
r

Subunits

Subunits Comment Organism
monomer 1 * 50000, about, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
HOT
-
Mus musculus
HOT
-
Homo sapiens
HOT
-
Rattus norvegicus
transhydrogenase, hydroxy acid-oxo acid
-
Homo sapiens
transhydrogenase, hydroxy acid-oxo acid
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mus musculus
30
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
-
Rattus norvegicus
7.1
-
assay at Mus musculus
7.1
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
additional information enzymatic activity is unaffected by NAD+ or NADP+ up to 1 mM Homo sapiens
additional information enzymatic activity is unaffected by NAD+ or NADP+ up to 1 mM Rattus norvegicus
additional information no requirement for NAD+ or NADP+ Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.075
-
cyanide 30°C, pH 7.1 Rattus norvegicus
0.75
-
1,10-phenanthroline 30°C, pH 7.1 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.075
-
IC50: 0.075 mM Rattus norvegicus cyanide
0.75
-
IC50: 0.75 mM Rattus norvegicus 1,10-phenanthroline