Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by EDTA and EGTA | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in HEK-293 cells as soluble protein | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | IC50: 0.75 mM | Rattus norvegicus | |
cyanide | IC50: 0.075 mM | Rattus norvegicus | |
additional information | no effect by EDTA and EGTA; no inhibition with EDTA, EGTA | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | no activity with L-isomer of 2-hydroxybutyrate, Km for succinate semialdehyde is below 0.01 mM, 30°C, pH 7.1 | Rattus norvegicus | |
0.01 | - |
Succinic semialdehyde | below, pH 7.1, 30°C | Rattus norvegicus | |
0.06 | - |
4-hydroxybutyrate | pH 7.1, 30°C | Rattus norvegicus | |
0.06 | - |
4-hydroxybutyrate | +/- 0.006 mM, 30°C, pH 7.1 | Rattus norvegicus | |
0.067 | - |
4-hydroxybutyrate | +/-0.012 mM, in overepressed HEK cells, in presence of 0.5 mM 2-oxoglutarate | Homo sapiens | |
0.067 | - |
4-hydroxybutyrate | pH 7.1, 30°C, recombinant enzyme | Mus musculus | |
0.077 | - |
2-oxoglutarate | +/-0.009 mM, in overexpressed HEK cells, in presence of 0.1 mM 4-hydroxybutyrate | Homo sapiens | |
0.077 | - |
2-oxoglutarate | pH 7.1, 30°C, recombinant enzyme | Mus musculus | |
0.085 | - |
2-oxoglutarate | pH 7.1, 30°C | Rattus norvegicus | |
0.085 | - |
2-oxoglutarate | +/-0.007 mM, 30°C, pH 7.1 | Rattus norvegicus | |
4.5 | - |
D-2-hydroxyglutarate | pH 7.1, 30°C | Rattus norvegicus | |
4.5 | - |
D-2-hydroxybutyrate | no activity with L-isomer, 30°C, pH 7.1 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | bi or trivalent, no stimulation with 0.01-0.05 mM Fe2+, Fe3+, Co2+, Ni2+, Mn2+, Zn2+, Mg2+ or Ca2+ | Rattus norvegicus | |
additional information | not affected by 0.5 mM Fe2+, Fe3+, Co2+, Ni2+, Mn2+, and Zn2+, or 1 mM Mg2+ and Ca2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | 55000 | gel filtration | Rattus norvegicus |
45000 | 50000 | - |
Rattus norvegicus |
50000 | - |
1 * 50000, about, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybutyrate + 2-oxoglutarate | Homo sapiens | - |
D-2-hydroxyglutarate + succinate semialdehyde | - |
? | |
4-hydroxybutyrate + 2-oxoglutarate | Rattus norvegicus | - |
D-2-hydroxyglutarate + succinate semialdehyde | - |
? | |
4-hydroxybutyrate + 2-oxoglutarate | Rattus norvegicus | - |
succinic semialdehyde + D-2-hydroxyglutarate | - |
r | |
4-hydroxybutyrate + 2-oxoglutarate | Mus musculus | recombinant enzyme in HEK-293 cells | succinic semialdehyde + D-2-hydroxyglutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1300fold, partial from liver | Rattus norvegicus |
native enzyme from liver 1300fold by precipitation using PEG 6000, anion exchange and hydrophobic interaction chromatography, and gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | low enzyme activity | Rattus norvegicus | - |
heart | low enzyme activity | Rattus norvegicus | - |
heart | highest activity in liver and kidney and an intermediate activity in heart | Rattus norvegicus | - |
HEK-293 cell | mouse homologue expressed in | Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
kidney | highest activity in liver and kidney and an intermediate activity in heart | Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
liver | highest activity in liver and kidney and an intermediate activity in heart | Rattus norvegicus | - |
lung | very low enzyme activity | Rattus norvegicus | - |
additional information | tissue distribution, overview | Rattus norvegicus | - |
additional information | no activity in brain and skeletal muscle | Rattus norvegicus | - |
skeletal muscle | low enzyme activity | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0013 | - |
recombinant soluble enzyme in HEK-293 cells | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybutyrate + 2-oxoglutarate | - |
Homo sapiens | D-2-hydroxyglutarate + succinate semialdehyde | - |
? | |
4-hydroxybutyrate + 2-oxoglutarate | - |
Rattus norvegicus | D-2-hydroxyglutarate + succinate semialdehyde | - |
? | |
4-hydroxybutyrate + 2-oxoglutarate | - |
Mus musculus | succinic semialdehyde + D-2-hydroxyglutarate | - |
? | |
4-hydroxybutyrate + 2-oxoglutarate | - |
Rattus norvegicus | succinic semialdehyde + D-2-hydroxyglutarate | - |
r | |
4-hydroxybutyrate + 2-oxoglutarate | recombinant enzyme in HEK-293 cells | Mus musculus | succinic semialdehyde + D-2-hydroxyglutarate | - |
? | |
4-hydroxybutyrate + 2-oxoglutarate | stereospecific reaction, no activity with L-2-hydroxyglutarate | Rattus norvegicus | succinic semialdehyde + D-2-hydroxyglutarate | - |
r | |
D-2-hydroxybutyrate + 2-oxoglutarate | - |
Rattus norvegicus | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, about, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
HOT | - |
Mus musculus |
HOT | - |
Homo sapiens |
HOT | - |
Rattus norvegicus |
transhydrogenase, hydroxy acid-oxo acid | - |
Homo sapiens |
transhydrogenase, hydroxy acid-oxo acid | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Rattus norvegicus |
7.1 | - |
assay at | Mus musculus |
7.1 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzymatic activity is unaffected by NAD+ or NADP+ up to 1 mM | Homo sapiens | |
additional information | enzymatic activity is unaffected by NAD+ or NADP+ up to 1 mM | Rattus norvegicus | |
additional information | no requirement for NAD+ or NADP+ | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
cyanide | 30°C, pH 7.1 | Rattus norvegicus | |
0.75 | - |
1,10-phenanthroline | 30°C, pH 7.1 | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.075 | - |
IC50: 0.075 mM | Rattus norvegicus | cyanide | |
0.75 | - |
IC50: 0.75 mM | Rattus norvegicus | 1,10-phenanthroline |