Literature summary for 1.1.99.22 extracted from

Michizoe, J.; Ichinose, H.; Kamiya, N.; Maruyama, T.; Goto, M.
Functionalization of the cytochrome P450cam monooxygenase system in the cell-like aqueous compartments of water-in-oil emulsions (2005), J. Biosci. Bioeng., 99, 12-17.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21(DE3)pLysS as His-tag fusion protein	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli JM109	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity by anion exchange chromatography and Ni-affinity chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycerol + NAD+	-	Escherichia coli	dihydroxyacetone + NADH + H+	-	?
glycerol + NAD+	-	Escherichia coli JM109	dihydroxyacetone + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
GLD	-	Escherichia coli
glycerol dehydrogenase	-	Escherichia coli

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Release 2023.1 (January 2023)