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show all sequences of 1.1.99.21

Enhanced production of L-sorbose from D-sorbitol by improving the mRNA abundance of sorbitol dehydrogenase in Gluconobacter oxydans WSH-003

Xu, S.; Wang, X.; Du, G.; Zhou, J.; Chen, J.; Microb. Cell Fact. 13, 146 (2014)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the most prominent industrial method of producing L-sorbose is the biotransformation of D-sorbitol to L-sorbose in Gluconobacter species or Acetobacter species. L-sorbose is an important carbohydrate that is predominantly used as a starting material in the biosynthesis of L-ascorbic acid
Gluconobacter oxydans
Cloned(Commentary)
Commentary
Organism
gene sldhAB, enzyme overexpression in an industrial strain Gluconobacter oxydans WSH-003 with a strong promoter PtufB, subcloning in Escherichia coli strain JM109, quantitative real-time PCR expression analysis
Gluconobacter oxydans
Engineering
Amino acid exchange
Commentary
Organism
additional information
method evaluation and optimization for engineered L-sorbose production in Gluconobater oxydans by self-overexpressing the sldhAB gene in Gluconobacter oxydans strain WSH-003 with an optimal poly(A/T) tail under the constitutive promoter PtufB, the titer and the productivity of L-sorbose are enhanced by 36.3% and 25.0%, respectively, in a 1-L fermenter. Immobilization of Gluconobacter oxydans-sldhAB6 cells further improves the L-sorbose titer by 33.7% after 20 days of semi-continuous fed-batch fermentation. Immobilization of recombinant enzyme in calcium alginate beads, the L-sorbose titer is improved by 33.7% by the immobilization of sldhAB6 cells
Gluconobacter oxydans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the membrane-bound dehydrogenase locates on the outer surface of the cytoplasmic membrane. The oxidation products accumulate in the culture medium
Gluconobacter oxydans
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-sorbitol + acceptor
Gluconobacter oxydans
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
L-sorbose + reduced acceptor
the oxidation product accumulates in the culture medium
-
?
D-sorbitol + acceptor
Gluconobacter oxydans WSH-003
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
L-sorbose + reduced acceptor
the oxidation product accumulates in the culture medium
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
Q70JP0 AND Q70JN9
small and large subunits, SldB and SldA
-
Gluconobacter oxydans WSH-003
Q70JP0 AND Q70JN9
small and large subunits, SldB and SldA
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-sorbitol + acceptor
-
740961
Gluconobacter oxydans
L-sorbose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
740961
Gluconobacter oxydans
L-sorbose + reduced acceptor
the oxidation product accumulates in the culture medium
-
-
?
D-sorbitol + acceptor
-
740961
Gluconobacter oxydans WSH-003
L-sorbose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
740961
Gluconobacter oxydans WSH-003
L-sorbose + reduced acceptor
the oxidation product accumulates in the culture medium
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
-
Gluconobacter oxydans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Gluconobacter oxydans
Application (protein specific)
Application
Commentary
Organism
synthesis
the most prominent industrial method of producing L-sorbose is the biotransformation of D-sorbitol to L-sorbose in Gluconobacter species or Acetobacter species. L-sorbose is an important carbohydrate that is predominantly used as a starting material in the biosynthesis of L-ascorbic acid
Gluconobacter oxydans
Cloned(Commentary) (protein specific)
Commentary
Organism
gene sldhAB, enzyme overexpression in an industrial strain Gluconobacter oxydans WSH-003 with a strong promoter PtufB, subcloning in Escherichia coli strain JM109, quantitative real-time PCR expression analysis
Gluconobacter oxydans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
method evaluation and optimization for engineered L-sorbose production in Gluconobater oxydans by self-overexpressing the sldhAB gene in Gluconobacter oxydans strain WSH-003 with an optimal poly(A/T) tail under the constitutive promoter PtufB, the titer and the productivity of L-sorbose are enhanced by 36.3% and 25.0%, respectively, in a 1-L fermenter. Immobilization of Gluconobacter oxydans-sldhAB6 cells further improves the L-sorbose titer by 33.7% after 20 days of semi-continuous fed-batch fermentation. Immobilization of recombinant enzyme in calcium alginate beads, the L-sorbose titer is improved by 33.7% by the immobilization of sldhAB6 cells
Gluconobacter oxydans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the membrane-bound dehydrogenase locates on the outer surface of the cytoplasmic membrane. The oxidation products accumulate in the culture medium
Gluconobacter oxydans
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-sorbitol + acceptor
Gluconobacter oxydans
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
L-sorbose + reduced acceptor
the oxidation product accumulates in the culture medium
-
?
D-sorbitol + acceptor
Gluconobacter oxydans WSH-003
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
L-sorbose + reduced acceptor
the oxidation product accumulates in the culture medium
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-sorbitol + acceptor
-
740961
Gluconobacter oxydans
L-sorbose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
740961
Gluconobacter oxydans
L-sorbose + reduced acceptor
the oxidation product accumulates in the culture medium
-
-
?
D-sorbitol + acceptor
-
740961
Gluconobacter oxydans WSH-003
L-sorbose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
D-sorbitol is oxidized in the periplasm in a chemo-, regio-, and stereoselective manner to L-sorbose by the membrane-bound dehydrogenase
740961
Gluconobacter oxydans WSH-003
L-sorbose + reduced acceptor
the oxidation product accumulates in the culture medium
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
-
Gluconobacter oxydans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Gluconobacter oxydans
Other publictions for EC 1.1.99.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
1
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1
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1
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740006
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-
Structural insights into the b ...
Gluconobacter oxydans
Biochem. Eng. J.
114
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2016
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1
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2
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3
3
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741426
Kim
A highly efficient sorbitol de ...
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Sci. Rep.
6
33438
2016
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1
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2
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6
2
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1
1
4
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5
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1
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6
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2
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1
1
4
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1
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2
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6
1
1
1
-
1
1
1
-
-
-
2
2
-
1
1
740961
Xu
Enhanced production of L-sorbo ...
Gluconobacter oxydans, Gluconobacter oxydans WSH-003
Microb. Cell Fact.
13
146
2014
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1
1
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1
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1
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2
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4
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4
1
1
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1
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1
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4
1
1
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1
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724595
Soemphol
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Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
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1
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1
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684722
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A pyrroloquinoline quinine-dep ...
Gluconobacter oxydans
Arch. Biochem. Biophys.
477
206-210
2008
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1
4
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1
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685690
Soemphol
Distinct physiological roles o ...
Gluconobacter frateurii
Biosci. Biotechnol. Biochem.
72
842-850
2008
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1
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3
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1
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1
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1
1
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1
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2
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695746
Yang
Membrane-bound pyrroloquinolin ...
Gluconobacter oxydans, Gluconobacter oxydans M5
Appl. Environ. Microbiol.
74
5250-5253
2008
-
-
1
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-
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-
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1
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3
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1
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1
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2
1
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1
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1
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1
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672655
Toyama
Molecular properties of membra ...
Gluconobacter frateurii
Biosci. Biotechnol. Biochem.
69
1120-1129
2005
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654267
Matsushita
5-Keto-D-gluconate production ...
Gluconobacter oxydans
Appl. Environ. Microbiol.
69
1959-1966
2003
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1
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1
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1
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1
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8
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1
1
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1
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1
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1
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1
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8
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1
1
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389912
Shinagawa
-
Purification and characterizat ...
Gluconobacter oxydans
Agric. Biol. Chem.
46
135-141
1982
-
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3
-
1
1
1
4
1
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1
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2
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1
1
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1
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3
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3
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1
1
1
4
1
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1
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1
2
4
1
1
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1
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389913
Shinagawa
-
D-Sorbitol dehydrogenase from ...
Gluconobacter oxydans
Methods Enzymol.
89
141-145
1982
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1
1
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4
1
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4
1
1
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1
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3
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3
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1
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1
1
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1
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657542
Kinast
-
Vierstufige 1-Desoxynojirimyci ...
Gluconobacter oxydans
Angew. Chem.
93
799-800
1981
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