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Literature summary for 1.1.99.21 extracted from

  • Fredslund, F.; Otten, H.; Gemperlein, S.; Poulsen, J.C.; Carius, Y.; Kohring, G.W.; Lo Leggio, L.
    Structural characterization of the thermostable Bradyrhizobium japonicum D-sorbitol dehydrogenase (2016), Acta Crystallogr. Sect. F, 72, 846-852.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Gold (DE3) cells Bradyrhizobium japonicum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 1.4 M NaH2PO4/K2HPO4 pH 5.6 Bradyrhizobium japonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucitol + NAD+ Bradyrhizobium japonicum
-
 D-fructose + NADH + H+
-
 ?
D-glucitol + NAD+ Bradyrhizobium japonicum USDA110
-
 D-fructose + NADH + H+
-
 ?
L-glucitol + NAD+ Bradyrhizobium japonicum 90% conversion D-sorbose + NADH + H+
-
 ?
L-glucitol + NAD+ Bradyrhizobium japonicum USDA110 90% conversion D-sorbose + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bradyrhizobium japonicum
-
-
-
Bradyrhizobium japonicum USDA110
-
-
-

Purification (Commentary)

Purification (Comment) Organism
metal affinity column chromatography Bradyrhizobium japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucitol + NAD+
-
 Bradyrhizobium japonicum D-fructose + NADH + H+
-
 ?
D-glucitol + NAD+
-
 Bradyrhizobium japonicum USDA110 D-fructose + NADH + H+
-
 ?
L-glucitol + NAD+ 90% conversion Bradyrhizobium japonicum D-sorbose + NADH + H+
-
 ?
L-glucitol + NAD+ 90% conversion Bradyrhizobium japonicum USDA110 D-sorbose + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
SDH
-
 Bradyrhizobium japonicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
 melting temperature Bradyrhizobium japonicum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bradyrhizobium japonicum