Cloned (Comment) | Organism |
---|---|
in Escherichia coli, plasmids harboring the disrupted gene are introduced into Gluconobacter frateurii THD32 by electroporation | Gluconobacter frateurii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter frateurii | - |
THD32, wild type and mutant defective in FAD-SLDH | - |
Purification (Comment) | Organism |
---|---|
by cell disruption and ultracentrifugation | Gluconobacter frateurii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol + acceptor | production of L-sorbose is similar in the wild-type strain and the FAD-SLDH defective strain, the enzyme is induced by L-sorbose and by D-sorbitol, FAD-SLDH links preferably to the cyanide-insensitive terminal oxidase | Gluconobacter frateurii | L-sorbose + reduced acceptor | - |
? | |
D-sorbitol + acceptor | production of L-sorbose is similar in the wild-type strain and the FAD-SLDH defective strain, the enzyme is induced by L-sorbose and by D-sorbitol, FAD-SLDH links preferably to the cyanide-insensitive terminal oxidase | Gluconobacter frateurii THD32 | L-sorbose + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-sorbitol dehydrogenase | membrane-bound FAD-dependent D-sorbitol dehydrogenase FAD-SLDH | Gluconobacter frateurii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Gluconobacter frateurii |