BRENDA - Enzyme Database show
show all sequences of 1.1.99.21

5-Keto-D-gluconate production is catalyzed by a quinoprotein glycerol dehydrogenase, major polyol dehydrogenase, in Gluconobacter species

Matsushita, K.; Fujii, Y.; Ano, Y.; Toyama, H.; Shinjoh, M.; Tomiyama, N.; Miyazaki, T.; Sugisawa, T.; Hoshino, T.; Adachi, O.; Appl. Environ. Microbiol. 69, 1959-1966 (2003)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
enzyme disruption mutant, oxidation activity against D-arabitol, D-sorbitol, D-mannitol, ribitol, meso-erythritol and glycerol are diminished
Gluconobacter oxydans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
cytoplasmic membrane
Gluconobacter oxydans
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Gluconobacter oxydans
production of 5-keto-D-gluconate is solely dependent on enzyme
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
-
identical with glycerol dehydrogenase, EC1.1.99.22, and D-arabitol dehydrogenase, EC1.1.1.11
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + acceptor
-
654267
Gluconobacter oxydans
D-xylulose + reduced acceptor
-
-
-
?
D-gluconate + acceptor
-
654267
Gluconobacter oxydans
5-keto-D-gluconate + reduced acceptor
-
-
-
?
D-mannitol + acceptor
-
654267
Gluconobacter oxydans
D-fructose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
-
654267
Gluconobacter oxydans
L-sorbose + reduced acceptor
-
-
-
?
glycerol + acceptor
-
654267
Gluconobacter oxydans
dihydroxyacetone + reduced acceptor
-
-
-
?
meso-erythritol + acceptor
-
654267
Gluconobacter oxydans
L-erythrulose + reduced acceptor
-
-
-
?
additional information
production of 5-keto-D-gluconate is solely dependent on enzyme
654267
Gluconobacter oxydans
?
-
-
-
-
ribitol + acceptor
-
654267
Gluconobacter oxydans
? + reduced acceptor
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
assay at
Gluconobacter oxydans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
9
-
about 50% of activity at pH 5.0
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
acts in vitro as acceptor together with phenazine methosulfate
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
acts in vitro as acceptor together with phenazine methosulfate
Gluconobacter oxydans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
enzyme disruption mutant, oxidation activity against D-arabitol, D-sorbitol, D-mannitol, ribitol, meso-erythritol and glycerol are diminished
Gluconobacter oxydans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
cytoplasmic membrane
Gluconobacter oxydans
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Gluconobacter oxydans
production of 5-keto-D-gluconate is solely dependent on enzyme
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + acceptor
-
654267
Gluconobacter oxydans
D-xylulose + reduced acceptor
-
-
-
?
D-gluconate + acceptor
-
654267
Gluconobacter oxydans
5-keto-D-gluconate + reduced acceptor
-
-
-
?
D-mannitol + acceptor
-
654267
Gluconobacter oxydans
D-fructose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
-
654267
Gluconobacter oxydans
L-sorbose + reduced acceptor
-
-
-
?
glycerol + acceptor
-
654267
Gluconobacter oxydans
dihydroxyacetone + reduced acceptor
-
-
-
?
meso-erythritol + acceptor
-
654267
Gluconobacter oxydans
L-erythrulose + reduced acceptor
-
-
-
?
additional information
production of 5-keto-D-gluconate is solely dependent on enzyme
654267
Gluconobacter oxydans
?
-
-
-
-
ribitol + acceptor
-
654267
Gluconobacter oxydans
? + reduced acceptor
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
assay at
Gluconobacter oxydans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
9
-
about 50% of activity at pH 5.0
Gluconobacter oxydans
Other publictions for EC 1.1.99.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739825
Fredslund
Structural characterization of ...
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA110
Acta Crystallogr. Sect. F
72
846-852
2016
-
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1
1
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-
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4
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3
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1
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1
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1
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4
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1
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740006
Selvaraj
-
Structural insights into the b ...
Gluconobacter oxydans
Biochem. Eng. J.
114
244-256
2016
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1
1
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1
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1
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2
2
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1
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1
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2
2
-
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3
3
-
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741426
Kim
A highly efficient sorbitol de ...
Gluconobacter oxydans, Gluconobacter oxydans G624
Sci. Rep.
6
33438
2016
-
-
1
-
2
-
6
2
-
1
1
4
-
5
-
-
1
-
-
-
2
-
6
1
1
1
-
1
1
1
-
3
-
-
-
-
-
1
3
-
2
-
-
6
-
2
-
1
1
4
-
-
-
1
-
-
2
-
6
1
1
1
-
1
1
1
-
-
-
2
2
-
1
1
740961
Xu
Enhanced production of L-sorbo ...
Gluconobacter oxydans, Gluconobacter oxydans WSH-003
Microb. Cell Fact.
13
146
2014
-
1
1
-
1
-
-
-
1
-
-
2
-
4
-
-
-
-
-
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-
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4
1
1
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1
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1
1
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1
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1
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2
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4
1
1
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1
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724595
Soemphol
Characterization of genes invo ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
76
1497-1505
2012
-
-
-
-
-
-
-
-
1
-
-
2
-
5
-
-
-
-
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2
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1
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1
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1
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2
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2
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-
-
1
-
-
1
-
-
684722
Yang
A pyrroloquinoline quinine-dep ...
Gluconobacter oxydans
Arch. Biochem. Biophys.
477
206-210
2008
-
-
-
-
-
-
1
4
-
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1
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1
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1
2
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1
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1
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1
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4
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1
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1
2
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685690
Soemphol
Distinct physiological roles o ...
Gluconobacter frateurii
Biosci. Biotechnol. Biochem.
72
842-850
2008
-
-
1
-
-
-
-
-
-
-
-
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3
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1
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2
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1
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1
1
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1
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2
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-
695746
Yang
Membrane-bound pyrroloquinolin ...
Gluconobacter oxydans, Gluconobacter oxydans M5
Appl. Environ. Microbiol.
74
5250-5253
2008
-
-
1
-
-
-
-
-
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1
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3
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1
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1
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2
1
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1
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1
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1
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1
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2
1
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-
-
-
-
672655
Toyama
Molecular properties of membra ...
Gluconobacter frateurii
Biosci. Biotechnol. Biochem.
69
1120-1129
2005
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-
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-
-
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3
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-
654267
Matsushita
5-Keto-D-gluconate production ...
Gluconobacter oxydans
Appl. Environ. Microbiol.
69
1959-1966
2003
-
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
1
-
1
-
-
-
-
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-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
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-
-
8
-
-
-
-
-
1
1
-
-
-
-
-
-
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-
389912
Shinagawa
-
Purification and characterizat ...
Gluconobacter oxydans
Agric. Biol. Chem.
46
135-141
1982
-
-
-
-
-
3
-
1
1
1
4
1
-
1
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-
1
-
-
-
1
2
4
1
1
-
-
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1
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3
-
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3
-
-
3
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-
1
1
1
4
1
-
-
-
1
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1
2
4
1
1
-
-
-
1
-
-
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-
-
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-
389913
Shinagawa
-
D-Sorbitol dehydrogenase from ...
Gluconobacter oxydans
Methods Enzymol.
89
141-145
1982
-
-
-
-
-
-
-
1
1
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4
1
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1
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1
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-
1
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4
1
1
-
-
-
1
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3
-
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-
3
-
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-
1
1
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4
1
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1
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-
1
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4
1
1
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-
1
-
-
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-
-
-
-
-
-
657542
Kinast
-
Vierstufige 1-Desoxynojirimyci ...
Gluconobacter oxydans
Angew. Chem.
93
799-800
1981
-
1
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1
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1
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1
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1
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1
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1
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