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Literature summary for 1.1.99.20 extracted from
- Purification and characterization of constitutive polyethylene glycol (PEG) dehydrogenase of a PEG 4000-utilizing Flavobacterium sp. No. 203 (1989), J. Ferment. Bioeng., 67, 324-330.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | - |
Flavobacterium sp. |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,4-benzoquinone | at 0.01-1 mM inhibitor; competitive to 2,6-dichlorophenolindophenol | Flavobacterium sp. | |
| arsenite | - |
Flavobacterium sp. | |
| Cd2+ | - |
Flavobacterium sp. | |
| Co2+ | - |
Flavobacterium sp. | |
| Cu2+ | at 1 mM; competitive | Flavobacterium sp. | |
| Fe3+ | - |
Flavobacterium sp. | |
| Hg2+ | at 1 mM; competitive | Flavobacterium sp. | |
| hydrazine | weak | Flavobacterium sp. | |
| hydroxylamine | weak | Flavobacterium sp. | |
| Mn2+ | - |
Flavobacterium sp. | |
| monoiodoacetate | weak | Flavobacterium sp. | |
| Ni2+ | - |
Flavobacterium sp. | |
| p-chloromercuribenzoate | - |
Flavobacterium sp. | |
| Semicarbazide | weak | Flavobacterium sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
PEG-400 | - |
Flavobacterium sp. | |
| 1.7 | - |
PEG-1000 | - |
Flavobacterium sp. | |
| 2.8 | - |
PEG-4000 | - |
Flavobacterium sp. | |
| 5.9 | - |
PEG-6000 | - |
Flavobacterium sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Flavobacterium sp. | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
4 * 57000, Flavobacterium sp. No.203, SDS-PAGE | Flavobacterium sp. |
| 220000 | - |
strain No.203, gel filtration | Flavobacterium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium sp. | - |
strains No.202 to 205 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| strain No. 203, by precipitation with ammonium sulfate, solubilization with benzalkonium chloride, chromatography with DEAE-Toyopearl 650M and hydroxylapatite and gel filtration on Toyopearl HW-55 | Flavobacterium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Flavobacterium sp. |
| 2.59 | - |
- |
Flavobacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-dodecanol + acceptor | - |
Flavobacterium sp. | n-dodecanal + reduced acceptor | - |
? | |
| 1-hexanol + acceptor | - |
Flavobacterium sp. | n-hexanal + reduced acceptor | - |
? | |
| 1-octanol + acceptor | - |
Flavobacterium sp. | n-octanal + reduced acceptor | - |
? | |
| 2-carboxybenzaldehyde + acceptor | - |
Flavobacterium sp. | 2-carboxybenzoic acid + reduced acceptor | - |
? | |
| acetaldehyde + acceptor + H2O | - |
Flavobacterium sp. | acetic acid + reduced acceptor | - |
? | |
| aliphatic aldehyde + acceptor + H2O | C-3 to C-7 | Flavobacterium sp. | carboxylic acid + reduced acceptor | - |
? | |
| aromatic alcohol + acceptor | - |
Flavobacterium sp. | aromatic aldehyde + reduced acceptor | - |
? | |
| aromatic aldehyde + acceptor + H2O | - |
Flavobacterium sp. | aromatic carboxylic acid + reduced acceptor | - |
? | |
| benzaldehyde + acceptor + H2O | - |
Flavobacterium sp. | benzoic acid + reduced acceptor | - |
? | |
| benzyl alcohol + acceptor | - |
Flavobacterium sp. | benzaldehyde + reduced acceptor | - |
? | |
| beta-phenethyl alcohol + acceptor | - |
Flavobacterium sp. | beta-phenethyl aldehyde + reduced acceptor | - |
? | |
| cinnamyl alcohol + acceptor | - |
Flavobacterium sp. | cinnamyl aldehyde + reduced acceptor | - |
? | |
| diethyleneglycol monoethylether | - |
Flavobacterium sp. | ? | - |
? | |
| diols + acceptor | except ethylene glycol, 1,2-and 1,3-propenediol | Flavobacterium sp. | dialdehydes + reduced acceptor | - |
? | |
| diols + acceptor | diethylene glycol and triethylene glycol are only poor substrates | Flavobacterium sp. | dialdehydes + reduced acceptor | - |
? | |
| diols + acceptor | overview: with primary alcoholic groups | Flavobacterium sp. | dialdehydes + reduced acceptor | - |
? | |
| ethylene glycol monoethylether | - |
Flavobacterium sp. | ? | - |
? | |
| ethyleneglycol monophenylether | - |
Flavobacterium sp. | ? | - |
? | |
| ethyleneglycolmono-n-butylether | - |
Flavobacterium sp. | ? | - |
? | |
| additional information | the enzyme shows no activity toward ethylene glycol, glycerol, secondary alcohols and sugar alcohols | Flavobacterium sp. | ? | - |
? | |
| PEG-1000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| PEG-200 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| PEG-20000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| PEG-300 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| PEG-400 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| PEG-4000 + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| PEG-6000 + acceptor | most active substrate of all PEGs | Flavobacterium sp. | ? | - |
? | |
| polyethylene glycol + acceptor | - |
Flavobacterium sp. | ? | - |
? | |
| primary alcohol + acceptor | C-3 to C-12 | Flavobacterium sp. | aldehyde + reduced acceptor | - |
? | |
| primary alcohol + acceptor | methanol, glycerol, secondary alcohols and sugar alcohols are no substrates | Flavobacterium sp. | aldehyde + reduced acceptor | - |
? | |
| primary alcohol + acceptor | 2,6-dichlorophenolindophenol is the acceptor in vitro | Flavobacterium sp. | aldehyde + reduced acceptor | - |
? | |
| primary alcohol + acceptor | coenzyme Q1 and Q2 can act as acceptors | Flavobacterium sp. | aldehyde + reduced acceptor | - |
? | |
| tetraethylene glycol + acceptor | - |
Flavobacterium sp. | tetraethylene glycol aldehyde + reduced acceptor | - |
? | |
| trans-cinnamaldehyde + acceptor | - |
Flavobacterium sp. | trans-cinnamic acid + reduced acceptor | - |
? | |
| vanillin aldehyde + acceptor + H2O | - |
Flavobacterium sp. | vanillic acid + reduced acceptor | - |
? | |
| vannilyl alcohol + acceptor | - |
Flavobacterium sp. | vannilyl aldehyde + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 57000, Flavobacterium sp. No.203, SDS-PAGE | Flavobacterium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Flavobacterium sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | 50 | about half-maximal activity at 18°C and 50°C | Flavobacterium sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable below | Flavobacterium sp. |
| 60 | - |
78% loss of activity | Flavobacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Flavobacterium sp. |
| 8 | - |
- |
Flavobacterium sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | about 75% of maximal activity at pH 6.5 and pH 8.5 | Flavobacterium sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | stable | Flavobacterium sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | electron acceptor | Flavobacterium sp. | |
| coenzyme Q1 | electron acceptor | Flavobacterium sp. | |
| coenzyme Q2 | electron acceptor | Flavobacterium sp. | |
| additional information | nitroblue tetrazolium, cytochrome c, ferricyanide, NADP+, NAD+, and coenzyme Q6 and Q10 do not serve as electron acceptors | Flavobacterium sp. | |
| pyrroloquinoline quinone | prosthetic group, noncovalently bound to the enzyme | Flavobacterium sp. | |
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