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Literature summary for 1.1.99.18 extracted from

  • Jensen, U.; Mohammad-Beigi, H.; Shipovskov, S.; Sutherland, D.; Ferapontova, E.
    Activation of cellobiose dehydrogenase bioelectrocatalysis by carbon nanoparticles (2019), ChemElectroChem, 6, 5032-5040 .
No PubMed abstract available
Search for more literature for 1.1.99.18

Application

Application Comment Organism
biofuel production application in enzymatic fuel cells is limited by their relatively low activity with this substrate Humicola insolens
biofuel production application in enzymatic fuel cells is limited by their relatively low activity with this substrate Thermothelomyces fergusii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae Humicola insolens
expression in Pichia pastoris X-33 cells Thermothelomyces fergusii

Protein Variants

Protein Variants Comment Organism
C285Y mutation causes a 5.6time decrease in KM for glucose and a decreased affinity for the other disaccharides. Overall catalytic efficiency increases 6.7times as compared to the wild type enzyme Humicola insolens
C291Y mutation causes a 8.3times decrease in KM for glucose and a decreased affinity for the other disaccharides Thermothelomyces fergusii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
 cellobiose pH 7.5, 25°C, wild-type enzyme Humicola insolens
0.0078
-
 cellobiose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
0.0205
-
 lactose pH 7.5, 25°C, wild-type enzyme Humicola insolens
0.036
-
 lactose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
0.06
-
 cellobiose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
0.28
-
 cellobiose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
0.44
-
 lactose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
1.14
-
 lactose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
2
-
 maltose pH 7.5, 25°C, wild-type enzyme Humicola insolens
2.7
-
 maltose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
5.2
-
 D-glucose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
9.3
-
 D-glucose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
22.8
-
 maltose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
27.8
-
 D-glucose pH 7.5, 25°C, wild-type enzyme Humicola insolens
81
-
 maltose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
88.2
-
 D-glucose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Humicola insolens
-
-
extracellular
-
 Thermothelomyces fergusii
-
-

Organism

Organism UniProt Comment Textmining
Humicola insolens Q9P8H5
-
-
Thermothelomyces fergusii E7D6B9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Humicola insolens
-
 Thermothelomyces fergusii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + cytochrome c
-
 Humicola insolens cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
cellobiose + cytochrome c
-
 Thermothelomyces fergusii cellobiono-1,5-lactone + reduced cytochrome c
-
 ?
D-glucose + cytochrome c
-
 Humicola insolens D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
D-glucose + cytochrome c
-
 Thermothelomyces fergusii D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Humicola insolens 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
lactose + cytochrome c
-
 Thermothelomyces fergusii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
maltose + cytochrome c
-
 Humicola insolens 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?
maltose + cytochrome c
-
 Thermothelomyces fergusii 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced cytochrome c
-
 ?

Synonyms

Synonyms Comment Organism
Cdh
-
 Humicola insolens
Cdh
-
 Thermothelomyces fergusii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.053
-
 maltose pH 7.5, 25°C, wild-type enzyme Humicola insolens
0.14
-
 maltose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
0.23
-
 D-glucose pH 7.5, 25°C, wild-type enzyme Humicola insolens
0.26
-
 cellobiose pH 7.5, 25°C, wild-type enzyme Humicola insolens
0.29
-
 D-glucose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
0.33
-
 lactose pH 7.5, 25°C, wild-type enzyme Humicola insolens
0.4
-
 maltose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
0.4
-
 maltose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
0.44
-
 cellobiose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
0.5
-
 lactose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
2
-
 cellobiose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
2.2
-
 D-glucose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
2.3
-
 lactose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
2.36
-
 lactose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
2.44
-
 cellobiose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
3.3
-
 D-glucose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Humicola insolens
7.4
-
-
 Thermothelomyces fergusii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0017
-
 maltose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
0.0083
-
 D-glucose pH 7.5, 25°C, wild-type enzyme Humicola insolens
0.0175
-
 maltose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
0.025
-
 D-glucose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
0.0265
-
 maltose pH 7.5, 25°C, wild-type enzyme Humicola insolens
0.0558
-
 D-glucose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
0.148
-
 maltose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
0.355
-
 D-glucose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
0.44
-
 lactose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
1.6
-
 cellobiose pH 7.5, 25°C, mutant enzyme C285Y Humicola insolens
5.4
-
 lactose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
16.1
-
 lactose pH 7.5, 25°C, wild-type enzyme Humicola insolens
40.6
-
 cellobiose pH 7.5, 25°C, mutant enzyme C291Y Thermothelomyces fergusii
63.8
-
 lactose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii
68.4
-
 cellobiose pH 7.5, 25°C, wild-type enzyme Humicola insolens
256
-
 cellobiose pH 7.5, 25°C, wild-type enzyme Thermothelomyces fergusii