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Literature summary for 1.1.99.18 extracted from

  • Bao, W.; Usha, S.N.; Renganathan, V.
    Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungus Phanerochaete chrysosporium (1993), Arch. Biochem. Biophys., 300, 705-713 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
ferricytochrome c pH 4.5, 22°C Phanerodontia chrysosporium
0.025
-
cellobiose pH 4.5, 22°C Phanerodontia chrysosporium
0.055
-
cellopentaose pH 4.5, 22°C Phanerodontia chrysosporium
0.061
-
cellotetraose pH 4.5, 22°C Phanerodontia chrysosporium
0.062
-
cellotriose pH 4.5, 22°C Phanerodontia chrysosporium
0.63
-
lactose pH 4.5, 22°C Phanerodontia chrysosporium

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the ferric enzyme does not bind azide or cyanide, implying that the heme iron is probably hexacoordinate Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
gel filtration Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content 9.4% Phanerodontia chrysosporium

Purification (Commentary)

Purification (Comment) Organism
-
Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.3
-
pH 4.5, 22°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + ferricytochrome c cellobiose is the preferred substrate and cytochrome c is the preferred electron acceptor Phanerodontia chrysosporium cellobiono-1,5-lactone + ferrocytochrome c
-
?
cellopentaose + ferricytochrome c
-
Phanerodontia chrysosporium cellopentaono-1,5-lactone + ferrocytochrome c
-
?
cellotetraose + ferricytochrome c
-
Phanerodontia chrysosporium cellotetraono-1,5-lactone + ferrocytochrome c
-
?
cellotriose + ferricytoferricytochrome c chrome c
-
Phanerodontia chrysosporium cellotriono-1,5-lactone + ferrocytochrome c
-
?
lactose + ferricytochrome c
-
Phanerodontia chrysosporium 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ferrocytochrome c
-
?
additional information electron acceptors are cytochrome c, benzoquinones, dichlorophenolindophenol and Mn3+. In the absence of these electron acceptors, oxygen serves as a poor electron acceptor and is reduced to H2O2 Phanerodontia chrysosporium ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 90000, SDS-PAGE Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
Cdh
-
Phanerodontia chrysosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20.5
-
ferricytochrome c pH 4.5, 22°C Phanerodontia chrysosporium
20.7
-
cellotetraose pH 4.5, 22°C Phanerodontia chrysosporium
21.5
-
cellopentaose pH 4.5, 22°C Phanerodontia chrysosporium
24
-
cellobiose pH 4.5, 22°C Phanerodontia chrysosporium
24
-
cellotriose pH 4.5, 22°C Phanerodontia chrysosporium
28.8
-
lactose pH 4.5, 22°C Phanerodontia chrysosporium

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10
-
Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
cytochrome b one heme b and one FAD per monomer. The native ferric form of the enzyme has absorption maxima at 420, 529, and 570 nm. The ferric enzyme does not bind azide or cyanide, implying that the heme iron is probably hexacoordinate Phanerodontia chrysosporium
FAD one heme b and one FAD per monomer Phanerodontia chrysosporium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
46
-
lactose pH 4.5, 22°C Phanerodontia chrysosporium
339
-
cellotetraose pH 4.5, 22°C Phanerodontia chrysosporium
387
-
cellotriose pH 4.5, 22°C Phanerodontia chrysosporium
391
-
cellopentaose pH 4.5, 22°C Phanerodontia chrysosporium
960
-
cellobiose pH 4.5, 22°C Phanerodontia chrysosporium
17083
-
ferricytochrome c pH 4.5, 22°C Phanerodontia chrysosporium