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Literature summary for 1.1.99.18 extracted from

  • Sulej, J.; Janusz, G.; Osinska-Jaroszuk, M.; Malek, P.; Mazur, A.; Komaniecka, I.; Choma, A.; Rogalski, J.
    Characterization of cellobiose dehydrogenase and its FAD-domain from the ligninolytic basidiomycete Pycnoporus sanguineus (2013), Enzyme Microb. Technol., 53, 427-437.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 91% residual activity at 100 mM Trametes sanguinea
Cu2+ 4% residual activity at 100 mM Trametes sanguinea
CuSO4 86% residual activity at 100 mM Trametes sanguinea
EDTA 59% residual activity at 100 mM Trametes sanguinea
imidazole 48% residual activity at 100 mM Trametes sanguinea
KCN 28% residual activity at 100 mM Trametes sanguinea
MgSO4 95% residual activity at 100 mM Trametes sanguinea
MnSO4 88% residual activity at 100 mM Trametes sanguinea
additional information not inhibited by NaN3, NaBr, NaCl, MgCl2, MnCl2, Na2SO4, and ZnSO4 Trametes sanguinea
NaF 55% residual activity at 100 mM Trametes sanguinea
NaNO3 92% residual activity at 100 mM Trametes sanguinea
potassium fluoride 63% residual activity at 100 mM Trametes sanguinea
potassium iodide 79% residual activity at 100 mM Trametes sanguinea
SDS 5% residual activity at 100 mM Trametes sanguinea
Zn2+ 86% residual activity at 100 mM Trametes sanguinea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
cellobiose at pH 4.5 and 30°C Trametes sanguinea
0.73
-
lactose at pH 4.5 and 30°C Trametes sanguinea
60.7
-
maltose at pH 4.5 and 30°C Trametes sanguinea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
113400
-
-
Trametes sanguinea

Organism

Organism UniProt Comment Textmining
Trametes sanguinea S5RVR8
-
-
Trametes sanguinea FCL199 S5RVR8
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Sephadex G-50 gel filtration Trametes sanguinea

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.5
-
culture filtrate, with lactose and cytochrome c as substrates, at pH 4.5 and 30°C Trametes sanguinea
32.3
-
culture filtrate, with lactose and 2,6-dichlorophenolindophenol as substrates, at pH 4.5 and 30°C Trametes sanguinea
1416
-
after 91.4fold purification, with lactose and cytochrome c as substrates, at pH 4.5 and 30°C Trametes sanguinea
1770
-
after 54.7fold purification, with lactose and ,6-dichlorophenolindophenol as substrates, at pH 4.5 and 30°C Trametes sanguinea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenolindophenol
-
Trametes sanguinea cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
cellobiose + 2,6-dichlorophenolindophenol
-
Trametes sanguinea FCL199 cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
lactose + 2,6-dichlorophenolindophenol
-
Trametes sanguinea lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
lactose + 2,6-dichlorophenolindophenol
-
Trametes sanguinea FCL199 lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
lactose + cytochrome c
-
Trametes sanguinea lactono-1,5-lactone + reduced cytochrome c
-
?
lactose + cytochrome c
-
Trametes sanguinea FCL199 lactono-1,5-lactone + reduced cytochrome c
-
?
maltose + 2,6-dichlorophenolindophenol
-
Trametes sanguinea maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + 2,6-dichlorophenolindophenol
-
Trametes sanguinea FCL199 maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
? x * 113400, calculated from amino acid sequence Trametes sanguinea

Synonyms

Synonyms Comment Organism
Cdh
-
Trametes sanguinea
CDH1
-
Trametes sanguinea
cellobiose (acceptor) 1-oxidoreductase
-
Trametes sanguinea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
with 2,6-dichlorophenolindophenol as cosubstrate Trametes sanguinea
70
-
with cytochrome c as cosubstrate Trametes sanguinea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 more than 50% activity between 50 and 80°C Trametes sanguinea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Trametes sanguinea

pH Range

pH Minimum pH Maximum Comment Organism
3 6 more than 50% activity between pH 3.0 and 6.0 Trametes sanguinea

Cofactor

Cofactor Comment Organism Structure
FAD
-
Trametes sanguinea
heme
-
Trametes sanguinea

pI Value

Organism Comment pI Value Maximum pI Value
Trametes sanguinea isoelectric focusing
-
4.2