Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 91% residual activity at 100 mM | Trametes sanguinea | |
Cu2+ | 4% residual activity at 100 mM | Trametes sanguinea | |
CuSO4 | 86% residual activity at 100 mM | Trametes sanguinea | |
EDTA | 59% residual activity at 100 mM | Trametes sanguinea | |
imidazole | 48% residual activity at 100 mM | Trametes sanguinea | |
KCN | 28% residual activity at 100 mM | Trametes sanguinea | |
MgSO4 | 95% residual activity at 100 mM | Trametes sanguinea | |
MnSO4 | 88% residual activity at 100 mM | Trametes sanguinea | |
additional information | not inhibited by NaN3, NaBr, NaCl, MgCl2, MnCl2, Na2SO4, and ZnSO4 | Trametes sanguinea | |
NaF | 55% residual activity at 100 mM | Trametes sanguinea | |
NaNO3 | 92% residual activity at 100 mM | Trametes sanguinea | |
potassium fluoride | 63% residual activity at 100 mM | Trametes sanguinea | |
potassium iodide | 79% residual activity at 100 mM | Trametes sanguinea | |
SDS | 5% residual activity at 100 mM | Trametes sanguinea | |
Zn2+ | 86% residual activity at 100 mM | Trametes sanguinea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
cellobiose | at pH 4.5 and 30°C | Trametes sanguinea | |
0.73 | - |
lactose | at pH 4.5 and 30°C | Trametes sanguinea | |
60.7 | - |
maltose | at pH 4.5 and 30°C | Trametes sanguinea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
113400 | - |
- |
Trametes sanguinea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes sanguinea | S5RVR8 | - |
- |
Trametes sanguinea FCL199 | S5RVR8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Sephadex G-50 gel filtration | Trametes sanguinea |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.5 | - |
culture filtrate, with lactose and cytochrome c as substrates, at pH 4.5 and 30°C | Trametes sanguinea |
32.3 | - |
culture filtrate, with lactose and 2,6-dichlorophenolindophenol as substrates, at pH 4.5 and 30°C | Trametes sanguinea |
1416 | - |
after 91.4fold purification, with lactose and cytochrome c as substrates, at pH 4.5 and 30°C | Trametes sanguinea |
1770 | - |
after 54.7fold purification, with lactose and ,6-dichlorophenolindophenol as substrates, at pH 4.5 and 30°C | Trametes sanguinea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenolindophenol | - |
Trametes sanguinea | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellobiose + 2,6-dichlorophenolindophenol | - |
Trametes sanguinea FCL199 | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + 2,6-dichlorophenolindophenol | - |
Trametes sanguinea | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + 2,6-dichlorophenolindophenol | - |
Trametes sanguinea FCL199 | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
lactose + cytochrome c | - |
Trametes sanguinea | lactono-1,5-lactone + reduced cytochrome c | - |
? | |
lactose + cytochrome c | - |
Trametes sanguinea FCL199 | lactono-1,5-lactone + reduced cytochrome c | - |
? | |
maltose + 2,6-dichlorophenolindophenol | - |
Trametes sanguinea | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + 2,6-dichlorophenolindophenol | - |
Trametes sanguinea FCL199 | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 113400, calculated from amino acid sequence | Trametes sanguinea |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Trametes sanguinea |
CDH1 | - |
Trametes sanguinea |
cellobiose (acceptor) 1-oxidoreductase | - |
Trametes sanguinea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
with 2,6-dichlorophenolindophenol as cosubstrate | Trametes sanguinea |
70 | - |
with cytochrome c as cosubstrate | Trametes sanguinea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | more than 50% activity between 50 and 80°C | Trametes sanguinea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Trametes sanguinea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | more than 50% activity between pH 3.0 and 6.0 | Trametes sanguinea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trametes sanguinea | |
heme | - |
Trametes sanguinea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trametes sanguinea | isoelectric focusing | - |
4.2 |