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Literature summary for 1.1.99.18 extracted from

  • Sulej, J.; Janusz, G.; Osi?ska-Jaroszuk, M.; Rachubik, P.; Mazur, A.; Komaniecka, I.; Choma, A.; Rogalski, J.
    Characterization of cellobiose dehydrogenase from a biotechnologically important Cerrena unicolor strain (2015), Appl. Biochem. Biotechnol., 176, 1638-1658.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.18

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.175
-
 cellobiose with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor
0.285
-
 cellobiose with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor
1.85
-
 lactose with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor
5.241
-
 lactose with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 1 * 97000, SDS-PAGE Cerrena unicolor

Organism

Organism UniProt Comment Textmining
Cerrena unicolor S5R9V1
-
-
Cerrena unicolor FCL139 S5R9V1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is glycosylated (8.2%) mainly with mannose and glucosamine residues Cerrena unicolor

Purification (Commentary)

Purification (Comment) Organism
ammoinium sulfate precipitation, DEAE-Sepharose column chromatography, Sephadex G-50 gel filtration, and lactose-CPG column chromatography Cerrena unicolor

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 culture filtrate, at pH 4.5 and 30°C Cerrena unicolor
14.4
-
 after 73.41fold purification, at pH 4.5 and 30°C Cerrena unicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenolindophenol
-
 Cerrena unicolor cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
cellobiose + 2,6-dichlorophenolindophenol
-
 Cerrena unicolor FCL139 cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
cellobiose + ferricytochrome c
-
 Cerrena unicolor cellobiono-1,5-lactone + ferrocytochrome c
-
 ?
cellobiose + ferricytochrome c
-
 Cerrena unicolor FCL139 cellobiono-1,5-lactone + ferrocytochrome c
-
 ?
lactose + 2,6-dichlorophenolindophenol
-
 Cerrena unicolor lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
lactose + 2,6-dichlorophenolindophenol
-
 Cerrena unicolor FCL139 lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
lactose + ferricytochrome c
-
 Cerrena unicolor lactono-1,5-lactone + ferrocytochrome c
-
 ?
lactose + ferricytochrome c
-
 Cerrena unicolor FCL139 lactono-1,5-lactone + ferrocytochrome c
-
 ?
additional information the enzyme is unable to oxidize glucose Cerrena unicolor ?
-
 ?
additional information the enzyme is unable to oxidize glucose Cerrena unicolor FCL139 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 97000, SDS-PAGE Cerrena unicolor

Synonyms

Synonyms Comment Organism
Cdh
-
 Cerrena unicolor
CDH1
-
 Cerrena unicolor
cellobiose [acceptor] 1-oxidoreductase
-
 Cerrena unicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Cerrena unicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 90 complete loss of enzyme activity is recorded at 90°C after heat exposure for 30 min and at 50°C after 12 h Cerrena unicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.86
-
 cellobiose with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor
19.06
-
 cellobiose with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor
19.46
-
 lactose with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor
20.86
-
 lactose with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Cerrena unicolor

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 more than 50% activity between pH 3.4 and 6.0 Cerrena unicolor

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 9 the enzyme is stable for 12 h at pH 3.0-6.0, losing 50% of its initial activity within approximately 3 h at pH 2.0 and 8.0. The enzyme is especially sensitive to pH values above 9.0 Cerrena unicolor

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Cerrena unicolor
heme b
-
 Cerrena unicolor

pI Value

Organism Comment pI Value Maximum pI Value
Cerrena unicolor isoelectric focusing
-
 4.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.98
-
 lactose with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor
10.52
-
 lactose with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor
66.18
-
 cellobiose with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor
108.9
-
 cellobiose with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C Cerrena unicolor