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Literature summary for 1.1.99.18 extracted from

  • Sygmund, C.; Santner, P.; Krondorfer, I.; Peterbauer, C.K.; Alcalde, M.; Nyanhongo, G.S.; Guebitz, G.M.; Ludwig, R.
    Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production (2013), Microb. Cell Fact., 12, 38.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain BJ5465 Thermothelomyces myriococcoides

Protein Variants

Protein Variants Comment Organism
N700S the kcat for oxygen turnover is increased in this mutant (4.5fold), but also substrate turnover. A 3fold increase of the kcat for cellobiose with alternative electron acceptors indicates that mutation N700S influences the oxidative- and reductive FAD half-reaction Thermothelomyces myriococcoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01147
-
2,6-dichlorophenol indophenol recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
0.022
-
1,4-benzoquinone recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
0.033
-
2,6-dichlorophenol indophenol mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
0.037
-
O2 recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
0.056
-
1,4-benzoquinone mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
0.073
-
O2 mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + 1,4-benzoquinone Thermothelomyces myriococcoides
-
cellobiono-1,5-lactone + reduced 1,4-benzoquinone
-
?
cellobiose + 2,6-dichlorophenol indophenol Thermothelomyces myriococcoides
-
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellobiose + O2 Thermothelomyces myriococcoides
-
cellobiono-1,5-lactone + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Thermothelomyces myriococcoides A9XK88
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose column chromatography and Q-Source column chromatography Thermothelomyces myriococcoides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
culture supernatant, at pH 6.0 and 30°C Thermothelomyces myriococcoides
8
-
after 3.6fold purification, at pH 6.0 and 30°C Thermothelomyces myriococcoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 1,4-benzoquinone
-
Thermothelomyces myriococcoides cellobiono-1,5-lactone + reduced 1,4-benzoquinone
-
?
cellobiose + 2,6-dichlorophenol indophenol
-
Thermothelomyces myriococcoides cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellobiose + O2
-
Thermothelomyces myriococcoides cellobiono-1,5-lactone + H2O2
-
?

Synonyms

Synonyms Comment Organism
Cdh
-
Thermothelomyces myriococcoides
cellobiose oxidase
-
Thermothelomyces myriococcoides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
O2 recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
0.19
-
O2 mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
4.1
-
2,6-dichlorophenol indophenol recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
12.4
-
2,6-dichlorophenol indophenol mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
13.4
-
1,4-benzoquinone mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
43
-
1,4-benzoquinone recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides

Cofactor

Cofactor Comment Organism Structure
FAD
-
Thermothelomyces myriococcoides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
O2 recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
2.6
-
O2 mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
185
-
1,4-benzoquinone recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
240
-
1,4-benzoquinone mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
360
-
2,6-dichlorophenol indophenol recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
375
-
2,6-dichlorophenol indophenol mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides