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Literature summary for 1.1.99.18 extracted from

  • Zhang, R.; Fan, Z.; Kasuga, T.
    Expression of cellobiose dehydrogenase from Neurospora crassa in Pichia pastoris and its purification and characterization (2011), Protein Expr. Purif., 75, 63-69.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X33 Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0518
-
cellobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.0814
-
cellotriose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.0902
-
cellotetraose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.1131
-
cellopentaose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.1224
-
cellohexaose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.2
-
lactose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.74
-
Galactobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
1.53
-
mannobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
2.81
-
xylobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
634
-
galactose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
726
-
glucose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
5051
-
maltose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
5051
-
xylose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
5051
-
mannose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
mature peptide, calculated from amino acid sequence Neurospora crassa
120000
-
SDS-PAGE Neurospora crassa
130000
-
gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-
Neurospora crassa FGSC 2489
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the recombinant enzyme contains approximately 20.83% carbohydrate Neurospora crassa

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenol indophenol best substrate Neurospora crassa cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellobiose + 2,6-dichlorophenol indophenol best substrate Neurospora crassa FGSC 2489 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellohexaose + 2,6-dichlorophenol indophenol
-
Neurospora crassa cellohexono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellohexaose + 2,6-dichlorophenol indophenol
-
Neurospora crassa FGSC 2489 cellohexono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellopentaose + 2,6-dichlorophenol indophenol
-
Neurospora crassa cellopentono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellotetraose + 2,6-dichlorophenol indophenol
-
Neurospora crassa cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellotetraose + 2,6-dichlorophenol indophenol
-
Neurospora crassa FGSC 2489 cellotetrono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
cellotriose + 2,6-dichlorophenol indophenol
-
Neurospora crassa cellotriono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
galactobiose + 2,6-dichlorophenol indophenol good substrate Neurospora crassa ? + reduced 2,6-dichlorophenol indophenol
-
?
galactose + 2,6-dichlorophenol indophenol weak substrate Neurospora crassa ? + reduced 2,6-dichlorophenol indophenol
-
?
glucose + 2,6-dichlorophenol indophenol weak substrate Neurospora crassa ? + reduced 2,6-dichlorophenol indophenol
-
?
lactose + 2,6-dichlorophenol indophenol good substrate Neurospora crassa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
lactose + 2,6-dichlorophenol indophenol good substrate Neurospora crassa FGSC 2489 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
maltose + 2,6-dichlorophenol indophenol
-
Neurospora crassa ? + reduced 2,6-dichlorophenol indophenol
-
?
mannobiose + 2,6-dichlorophenol indophenol good substrate Neurospora crassa ? + reduced 2,6-dichlorophenol indophenol
-
?
mannose + 2,6-dichlorophenol indophenol weak substrate Neurospora crassa ? + reduced 2,6-dichlorophenol indophenol
-
?
xylobiose + 2,6-dichlorophenol indophenol good substrate Neurospora crassa 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
xylobiose + 2,6-dichlorophenol indophenol good substrate Neurospora crassa FGSC 2489 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
?
xylose + 2,6-dichlorophenol indophenol weak substrate Neurospora crassa ? + reduced 2,6-dichlorophenol indophenol
-
?

Synonyms

Synonyms Comment Organism
CDH1
-
Neurospora crassa
cellobiose dehydrogenase
-
Neurospora crassa
cellobiose:[acceptor] 1-oxidoreductase
-
Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 70
-
Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
the half life for the recombinant enzyme at 60°C is 20 min Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
Galactobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
2.1
-
galactose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
2.1
-
mannobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
3.7
-
xylobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
4.6
-
xylose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
7.6
-
glucose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
10.2
-
mannose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
11.1
-
lactose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
20.5
-
maltose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
23.9
-
cellotetraose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
24
-
cellobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
24.7
-
cellohexaose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
24.78
-
cellotriose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
25.7
-
cellopentaose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 the CDH activity decreases very sharply below pH 4.5. The recombinant enzyme remains at 60.8% of the maximum CDH activity at pH 4.0, and at only 22.6% CDH activity at pH 3.5 Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
FAD
-
Neurospora crassa
heme
-
Neurospora crassa

pI Value

Organism Comment pI Value Maximum pI Value
Neurospora crassa mature peptide, calculated from amino acid sequence
-
6.7
Neurospora crassa isoelectric focusing
-
6.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00091
-
xylose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.002
-
mannose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.0033
-
galactose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.004
-
maltose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
0.01
-
glucose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
1.33
-
xylobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
1.39
-
mannobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
1.92
-
Galactobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
55.4
-
lactose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
202.1
-
cellohexaose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
227
-
cellopentaose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
265
-
cellotetraose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
305.6
-
cellotriose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa
461.4
-
cellobiose recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C Neurospora crassa