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Literature summary for 1.1.99.18 extracted from
- Amino acid substitution at the substrate-binding subsite alters the specificity of the Phanerochaete chrysosporium cellobiose dehydrogenase (2010), Biochem. Biophys. Res. Commun., 391, 1246-1250.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain KM71 | Phanerodontia chrysosporium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E279A | the mutation has no effect on the expression of the protein in Pichia pastoris but completely abolishes its enzymatic activity | Phanerodontia chrysosporium |
| E279D | the mutation has no effect on the expression of the protein in Pichia pastoris but completely abolishes its enzymatic activity | Phanerodontia chrysosporium |
| E279N | the mutation has no effect on the expression of the protein in Pichia pastoris but completely abolishes its enzymatic activity. The mutant retains most of its activity with cellobiose but was completely inactive with lactose | Phanerodontia chrysosporium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
cellobiose | purified wild type enzyme, pH and temperature not specified in the publication | Phanerodontia chrysosporium |  |
| 1.16 | - |
lactose | purified wild type enzyme, pH and temperature not specified in the publication | Phanerodontia chrysosporium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
approximately 100000 Da, SDS-PAGE | Phanerodontia chrysosporium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | Q01738 | - |
- |
| Phanerodontia chrysosporium RP78 | Q01738 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Resource Q column chromatography | Phanerodontia chrysosporium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
purified mutant enzyme E279N, pH and temperature not specified in the publication | Phanerodontia chrysosporium |
| 1.9 | - |
purified wild type enzyme, pH and temperature not specified in the publication | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + acceptor | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| cellobiose + acceptor | - |
Phanerodontia chrysosporium RP78 | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| lactose + acceptor | - |
Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
| lactose + acceptor | - |
Phanerodontia chrysosporium RP78 | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cdh | - |
Phanerodontia chrysosporium |
| cellobiose dehydrogenase | - |
Phanerodontia chrysosporium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Phanerodontia chrysosporium | |
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Release 2023.1 (January 2023)
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