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Literature summary for 1.1.99.18 extracted from

  • Nyanhongo, G.S.; Guebitz, G.; Sukyai, P.; Leitner, C.; Haltrich, D.; Ludwig, R.
    Oxidoreductases from Trametes spp. in biotechnology: a wealth of catalytic activity (2007), Food Technol. Biotechnol., 45, 250-268.
No PubMed abstract available

Application

Application Comment Organism
industry CDH is involved in the degradation of the two most prominent biopolymers, cellulose and lignin, CDH can produce both reagents needed for fenton chemistry, H2O2 and ferrous iron complexes, which may migrate into and disrupt the lignocellulose matrix Trametes versicolor
industry CDH is involved in the degradation of the two most prominent biopolymers, cellulose and lignin, CDH can produce both reagents needed for fenton chemistry, H2O2 and ferrous iron complexes, which may migrate into and disrupt the lignocellulose matrix Trametes hirsuta
industry CDH is involved in the degradation of the two most prominent biopolymers, cellulose and lignin, CDH can produce both reagents needed for fenton chemistry, H2O2 and ferrous iron complexes, which may migrate into and disrupt the lignocellulose matrix Trametes villosa
industry CDH is involved in the degradation of the two most prominent biopolymers, cellulose and lignin, CDH can produce both reagents needed for fenton chemistry, H2O2 and ferrous iron complexes, which may migrate into and disrupt the lignocellulose matrix Trametes pubescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
cellobiose at pH 4.5 Trametes hirsuta
0.12
-
cellobiose at pH 4.5 Trametes versicolor
0.21
-
cellobiose at pH 4.5 Trametes villosa
0.21
-
cellobiose at pH 4.5 Trametes pubescens
0.22
-
cellotetraose at pH 4.5 Trametes hirsuta
0.42
-
cellotetraose at pH 4.5 Trametes pubescens
0.46
-
cellopentaose at pH 4.5 Trametes pubescens
0.47
-
cellotetraose at pH 4.5 Trametes villosa
1.4
-
cellopentaose at pH 4.5 Trametes hirsuta
2.13
-
lactose at pH 4.5 Trametes hirsuta
2.4
-
lactose at pH 4.5 Trametes pubescens
3.7
-
lactose at pH 4.5 Trametes villosa
350
-
maltose at pH 4.5 Trametes villosa
390
-
maltose at pH 4.5 Trametes pubescens
890
-
D-glucose at pH 4.5 Trametes pubescens
1300
-
D-glucose at pH 4.5 Trametes villosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
SDS-PAGE Trametes versicolor
98000
-
SDS-PAGE Trametes villosa
100000
-
SDS-PAGE Trametes pubescens

Organism

Organism UniProt Comment Textmining
Trametes hirsuta
-
white-rot basidiomycota
-
Trametes pubescens
-
white-rot basidiomycota
-
Trametes versicolor
-
white-rot basidiomycota
-
Trametes villosa
-
white-rot basidiomycota
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichloroindophenol 2,6-dichlorophenol-indolophenol decolouration assay Trametes versicolor cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + 2,6-dichloroindophenol 2,6-dichlorophenol-indolophenol decolouration assay Trametes hirsuta cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + 2,6-dichloroindophenol 2,6-dichlorophenol-indolophenol decolouration assay Trametes villosa cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + 2,6-dichloroindophenol 2,6-dichlorophenol-indolophenol decolouration assay Trametes pubescens cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellopentaose + acceptor
-
Trametes hirsuta ?
-
?
cellopentaose + acceptor
-
Trametes pubescens ?
-
?
cellotetraose + acceptor
-
Trametes hirsuta ?
-
?
cellotetraose + acceptor
-
Trametes villosa ?
-
?
cellotetraose + acceptor
-
Trametes pubescens ?
-
?
D-glucose + 2,6-dichloroindophenol
-
Trametes villosa ?
-
?
D-glucose + 2,6-dichloroindophenol
-
Trametes pubescens ?
-
?
lactose + acceptor
-
Trametes pubescens ?
-
?
lactose + acceptor low activity with maltose and glucose Trametes villosa ?
-
?
lactose + acceptor no activity at maltose and glucose Trametes hirsuta ?
-
?
maltose + 2,6-dichloroindophenol
-
Trametes villosa ?
-
?
maltose + 2,6-dichloroindophenol
-
Trametes pubescens ?
-
?

Subunits

Subunits Comment Organism
monomer
-
Trametes versicolor
monomer
-
Trametes villosa
monomer
-
Trametes pubescens

Synonyms

Synonyms Comment Organism
cellobiose dehydrogenase CDH Trametes versicolor
cellobiose dehydrogenase CDH Trametes hirsuta
cellobiose dehydrogenase CDH Trametes villosa
cellobiose dehydrogenase CDH Trametes pubescens

Cofactor

Cofactor Comment Organism Structure
cytochrome b type heme Trametes versicolor
cytochrome b type heme Trametes hirsuta
cytochrome b type heme Trametes villosa
cytochrome b type heme Trametes pubescens
FAD
-
Trametes versicolor
FAD
-
Trametes hirsuta
FAD
-
Trametes villosa
FAD
-
Trametes pubescens

pI Value

Organism Comment pI Value Maximum pI Value
Trametes versicolor
-
-
4.2
Trametes villosa
-
-
4.3
Trametes pubescens
-
-
4.4