Any feedback?
Literature summary for 1.1.99.18 extracted from
- Cellobiose oxidase, purification and partial characterization of a hemoprotein from Sporotrichum pulverulentum (1978), Eur. J. Biochem., 90, 171-181.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2-bipyridine | 1 mM, 41% inhibition | Phanerodontia chrysosporium |  |
| Sodium azide | 1 mM, 19% inhibition | Phanerodontia chrysosporium | |
| Sodium cyanide | 1 mM, 70% inhibition | Phanerodontia chrysosporium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
sedimentation equilibrium centrifugation | Phanerodontia chrysosporium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + O2 | Phanerodontia chrysosporium | role in cellulose degradation | cellobiono-1,5-lactone + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | - |
white-rot fungus | - |
| Phanerodontia chrysosporium | - |
name for perfect stage of this fungus, imperfect stage is named Sporotrichum pulverulentum | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | binds to immobilized concanavalin A | Phanerodontia chrysosporium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 138fold purification | Phanerodontia chrysosporium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-beta-glucosylmannose + O2 | 50% of the activity with lactose | Phanerodontia chrysosporium | ? | - |
? | |
| cellobiose + O2 | 162% of the activity with lactose | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | - |
? | |
| cellobiose + O2 | enzyme consumes equimolar amounts of oxygen and cellobiose | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | - |
? | |
| cellobiose + O2 | readily oxidized, cellobiose and cellohexaose with almost same activity | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | - |
? | |
| cellobiose + O2 | role in cellulose degradation | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | - |
? | |
| cellodextrin + O2 | high activity towards cellodextrins | Phanerodontia chrysosporium | aldonic acid + H2O2 | - |
? | |
| cellodextrin + O2 | decreasing rapidity in the series from cellobiose to cellotetraose, but then with increasing size they are more rapidly oxidized | Phanerodontia chrysosporium | aldonic acid + H2O2 | - |
? | |
| galactose + O2 | 26% of the activity with lactose | Phanerodontia chrysosporium | ? | - |
? | |
| lactose + O2 | readily oxidized | Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2 | product is lactobionic acid | ? | |
| maltose + O2 | 22% of the activity with lactose | Phanerodontia chrysosporium | 4-O-beta-glucosyl-glucono-1,5-lactone + H2O2 | - |
? | |
| additional information | not as substrates: glucose, xylose, cellulose, galactobiose, gentiobiose, H2O2 not detected as product | Phanerodontia chrysosporium | ? | - |
? | |
| sophorose + O2 | 17% of the activity with lactose | Phanerodontia chrysosporium | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phanerodontia chrysosporium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Phanerodontia chrysosporium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.1 | 5.7 | pH 4.1: less than 50% of maximum activity, pH 5.7: less than 50% of maximum activity | Phanerodontia chrysosporium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | flavohemoprotein, one flavin per enzyme molecule | Phanerodontia chrysosporium | |
| flavin | flavin component probably is the sugar dehydrogenating unit in the enzyme | Phanerodontia chrysosporium | |
| heme b | flavohemoprotein | Phanerodontia chrysosporium | |
| heme b | heme component serves for the storage of electrons to be incorporated into molecular oxygen for direct reduction to water | Phanerodontia chrysosporium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
