BRENDA - Enzyme Database show
show all sequences of 1.1.99.14

Enzymatic characterization of Chlamydomonas reinhardtii glycolate dehydrogenase and its nearest proteobacterial homologue

Aboelmy, M.H.; Peterhansel, C.; Plant Physiol. Biochem. 79, 25-30 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Chlamydomonas reinhardtii
Inhibitors
Inhibitors
Commentary
Organism
Structure
CuSO4
complete inhibition at 2 mM
Chlamydomonas reinhardtii
EDTA
8% inhibition at 10 mM
Chlamydomonas reinhardtii
KCN
complete inhibition at 2 mM
Chlamydomonas reinhardtii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.21
-
glycolate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
0.45
-
D-lactate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chlamydomonas reinhardtii
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-lactate + 2,6-dichlorophenolindophenol
100% activity with D-lactate and 2,6-dichlorophenolindophenol
741190
Chlamydomonas reinhardtii
?
-
-
-
?
D-lactate + phenazine methosulfate
93% activity compared to 2,6-dichlorophenolindophenol
741190
Chlamydomonas reinhardtii
?
-
-
-
?
glycerate 3-phosphate + 2,6-dichlorophenolindophenol
2.9% activity compared to D-lactate
741190
Chlamydomonas reinhardtii
?
-
-
-
?
glycolate + 2,6-dichlorophenolindophenol
71.7% activity compared to D-lactate
741190
Chlamydomonas reinhardtii
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-lactate + 2,6-dichlorophenolindophenol
12.4% activity compared to D-lactate
741190
Chlamydomonas reinhardtii
?
-
-
-
?
additional information
no activity with formate, malate, acetate, FAD, FMN, NAD+, NADP+, or cytochrome c
741190
Chlamydomonas reinhardtii
?
-
-
-
-
succinate + 2,6-dichlorophenolindophenol
8.5% activity compared to D-lactate
741190
Chlamydomonas reinhardtii
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Chlamydomonas reinhardtii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.93
-
glycolate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
5.42
-
D-lactate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
5.42
-
L-lactate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Chlamydomonas reinhardtii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Chlamydomonas reinhardtii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CuSO4
complete inhibition at 2 mM
Chlamydomonas reinhardtii
EDTA
8% inhibition at 10 mM
Chlamydomonas reinhardtii
KCN
complete inhibition at 2 mM
Chlamydomonas reinhardtii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.21
-
glycolate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
0.45
-
D-lactate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-lactate + 2,6-dichlorophenolindophenol
100% activity with D-lactate and 2,6-dichlorophenolindophenol
741190
Chlamydomonas reinhardtii
?
-
-
-
?
D-lactate + phenazine methosulfate
93% activity compared to 2,6-dichlorophenolindophenol
741190
Chlamydomonas reinhardtii
?
-
-
-
?
glycerate 3-phosphate + 2,6-dichlorophenolindophenol
2.9% activity compared to D-lactate
741190
Chlamydomonas reinhardtii
?
-
-
-
?
glycolate + 2,6-dichlorophenolindophenol
71.7% activity compared to D-lactate
741190
Chlamydomonas reinhardtii
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-lactate + 2,6-dichlorophenolindophenol
12.4% activity compared to D-lactate
741190
Chlamydomonas reinhardtii
?
-
-
-
?
additional information
no activity with formate, malate, acetate, FAD, FMN, NAD+, NADP+, or cytochrome c
741190
Chlamydomonas reinhardtii
?
-
-
-
-
succinate + 2,6-dichlorophenolindophenol
8.5% activity compared to D-lactate
741190
Chlamydomonas reinhardtii
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Chlamydomonas reinhardtii
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.93
-
glycolate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
5.42
-
D-lactate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
5.42
-
L-lactate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Chlamydomonas reinhardtii
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
10.92
-
glycolate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
13
-
D-lactate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
10.92
-
glycolate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
13
-
D-lactate
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0 and 30°C
Chlamydomonas reinhardtii
Other publictions for EC 1.1.99.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741130
Ahmad
-
Improvement of biomass accumul ...
Synechocystis sp.
Plant Biotechnol. Rep.
10
269-276
2016
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740208
Dalal
A photorespiratory bypass incr ...
Camelina sativa
Biotechnol. Biofuels
8
175
2015
-
-
1
-
-
-
-
-
1
-
-
1
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741129
Noelke
The expression of a recombinan ...
Escherichia coli
Plant Biotechnol. J.
12
734-742
2014
-
-
1
-
-
-
1
-
2
-
-
1
-
4
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
741190
Aboelmy
Enzymatic characterization of ...
Chlamydomonas reinhardtii
Plant Physiol. Biochem.
79
25-30
2014
-
-
1
-
-
-
3
2
-
-
-
-
-
6
-
-
-
-
-
-
-
-
7
-
1
-
-
3
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
3
1
-
-
-
-
-
-
-
2
2
725738
Isobe
-
Characterization and applicati ...
Trichoderma harzianum, Trichoderma harzianum AIU 353
J. Mol. Catal. B
83
94-99
2012
-
-
-
-
-
-
17
4
-
-
5
2
-
2
-
-
1
-
-
-
2
-
14
1
1
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
17
-
4
-
-
5
2
-
-
-
1
-
-
2
-
14
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
700960
Eisenhut
The photorespiratory glycolate ...
Synechocystis sp. PCC 6803
Proc. Natl. Acad. Sci. USA
105
17199-17204
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688079
Niessen
Mitochondrial glycolate oxidat ...
Arabidopsis thaliana
J. Exp. Bot.
58
2709-2715
2007
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676615
Eisenhut
The plant-like C2 glycolate cy ...
Synechocystis sp.
Plant Physiol.
142
333-342
2006
-
-
1
-
1
-
-
-
-
-
-
1
-
5
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
656411
Bari
A glycolate dehydrogenase in t ...
Arabidopsis thaliana
J. Exp. Bot.
55
623-630
2004
-
-
1
-
-
-
-
-
2
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348290
Sallal
-
Effects of crude oil and hydro ...
Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 1405-1
Microbios
90
33-43
1997
1
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348289
Stabenau
-
Localization of glycolate dehy ...
Dunaliella primolecta, Dunaliella primolecta 183.80, Dunaliella salina
Planta
191
362-364
1993
-
-
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348287
Sallal
Localization of glycollate deh ...
Dunaliella salina
Planta
171
429-432
1987
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348288
Yokota
-
The mechanism of induction of ...
Euglena gracilis, Euglena gracilis Z
Agric. Biol. Chem.
51
665-670
1987
-
-
-
-
-
-
2
1
-
-
-
2
-
21
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
1
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
348286
Paul
Photorespiration in diatoms. T ...
Thalassiosira pseudonana
Arch. Microbiol.
101
115-120
1974
-
-
-
-
-
-
3
3
1
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
3
1
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
348285
Lord
Glycolate oxidoreductase in Es ...
Escherichia coli
Biochim. Biophys. Acta
267
227-237
1971
-
-
-
-
-
-
8
2
1
-
-
1
-
2
-
-
1
-
-
-
3
1
8
-
1
-
-
-
1
1
-
4
-
-
-
-
-
-
4
-
-
-
-
8
-
2
1
-
-
1
-
-
-
1
-
-
3
1
8
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-