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Literature summary for 1.1.99.13 extracted from

  • Zhang, J.F.; Yang, B.; Chen, W.; Chen, J.
    Purification and characterization of the glucoside 3-dehydrogenase produced by a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251 (2014), Appl. Biochem. Biotechnol., 172, 3913-3925.
    View publication on PubMed
Search for more literature for 1.1.99.13

Activating Compound

Activating Compound Comment Organism Structure
EDTA 106.8% activity at 2 mM Sphingobacterium faecium

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 98.1% residual activity at 2 mM Sphingobacterium faecium
Ca2+ 78.2% residual activity at 2 mM Sphingobacterium faecium
Cd2+ 60.6% residual activity at 2 mM Sphingobacterium faecium
Cu2+ 38.5% residual activity at 2 mM Sphingobacterium faecium
Fe2+ 52.6% residual activity at 2 mM Sphingobacterium faecium
Hg2+ complete inhibition at 2 mM Sphingobacterium faecium
Zn2+ 91.1% residual activity at 2 mM Sphingobacterium faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 D-glucose at pH 6.0 and 25°C Sphingobacterium faecium
1.7
-
 sucrose at pH 6.0 and 25°C Sphingobacterium faecium
2.1
-
 validoxylamine A at pH 6.0 and 25°C Sphingobacterium faecium

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 136.4% activity at 2 mM Sphingobacterium faecium
Co2+ 116.7% activity at 2 mM Sphingobacterium faecium
Mg2+ 107.3% activity at 2 mM Sphingobacterium faecium
Mn2+ 104.2% activity at 2 mM Sphingobacterium faecium
additional information not influenced by K+ Sphingobacterium faecium
Ni2+ 113.5% activity at 2 mM Sphingobacterium faecium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62300
-
-
 Sphingobacterium faecium
63500
-
 x * 63500, calculated from amino acid sequence Sphingobacterium faecium

Organism

Organism UniProt Comment Textmining
Sphingobacterium faecium
-
 ZJF-D6 CCTCC M 2013251
-
Sphingobacterium faecium DSM 11690
-
 ZJF-D6 CCTCC M 2013251
-

Purification (Commentary)

Purification (Comment) Organism
High Q IEX column chromatography, hydroxyapatite column chromatography, and Sephadex G 75 gel filtration Sphingobacterium faecium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.21
-
 crude enzyme, at pH 6.0 and 25°C Sphingobacterium faecium
7.54
-
 after 35.71fold purification, at pH 6.0 and 25°C Sphingobacterium faecium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 21.5% activity compared to D-glucose Sphingobacterium faecium 3-dehydro-2-deoxy-D-glucose + reduced 2,6-dichlorophenolindophenol
-
 ?
alpha-methyl-D-glucoside + 2,6-dichlorophenolindophenol 96% activity compared to D-glucose Sphingobacterium faecium methyl 3-dehydro-alpha-D-glucoside + reduced 2,6-dichlorophenolindophenol
-
 ?
cellobiose + 2,6-dichlorophenolindophenol 97.4% activity compared to D-glucose Sphingobacterium faecium 4-O-beta-D-xylo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol
-
 ?
D-fructose + 2,6-dichlorophenolindophenol 10.8% activity compared to D-glucose Sphingobacterium faecium ?
-
 ?
D-galactose + 2,6-dichlorophenolindophenol 63.5% activity compared to D-glucose Sphingobacterium faecium 3-dehydro-D-galactose + 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol
-
 Sphingobacterium faecium 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol
-
 Sphingobacterium faecium DSM 11690 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
-
 ?
D-xylose + 2,6-dichlorophenolindophenol 3.1% activity compared to D-glucose Sphingobacterium faecium ?
-
 ?
D-xylose + 2,6-dichlorophenolindophenol 3.1% activity compared to D-glucose Sphingobacterium faecium DSM 11690 ?
-
 ?
isomaltose + 2,6-dichlorophenolindophenol 55.5% activity compared to D-glucose Sphingobacterium faecium 6-O-alpha-D-xylo-hexopyranosyl-4-ulose-alpha-D-glucopyranose + reduced 2,6-dichlorophenolindophenol
-
 ?
L-arabinose + 2,6-dichlorophenolindophenol 4.4% activity compared to D-glucose Sphingobacterium faecium ?
-
 ?
L-arabinose + 2,6-dichlorophenolindophenol 4.4% activity compared to D-glucose Sphingobacterium faecium DSM 11690 ?
-
 ?
lactose + 2,6-dichlorophenolindophenol 89.1% activity compared to D-glucose Sphingobacterium faecium 4-O-beta-D-xylo-hexopyranosyl-4-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol
-
 ?
maltose + 2,6-dichlorophenolindophenol 58% activity compared to D-glucose Sphingobacterium faecium 4-O-alpha-D-ribo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol
-
 ?
maltose + 2,6-dichlorophenolindophenol 58% activity compared to D-glucose Sphingobacterium faecium DSM 11690 4-O-alpha-D-ribo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol
-
 ?
additional information enzyme activity is low with L-arabinose and D-xylose, and with 3-O-methyl-D-glucose, D-mannitol, D-mannose, sucralose and D-sorbitol giving no activity Sphingobacterium faecium ?
-
 ?
additional information enzyme activity is low with L-arabinose and D-xylose, and with 3-O-methyl-D-glucose, D-mannitol, D-mannose, sucralose and D-sorbitol giving no activity Sphingobacterium faecium DSM 11690 ?
-
 ?
sucrose + 2,6-dichlorophenolindophenol
-
 Sphingobacterium faecium 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced 2,6-dichlorophenolindophenol
-
 ?
sucrose + 2,6-dichlorophenolindophenol 46.5% activity compared to D-glucose Sphingobacterium faecium 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced 2,6-dichlorophenolindophenol
-
 ?
trehalose + 2,6-dichlorophenolindophenol 63.8% activity compared to D-glucose Sphingobacterium faecium ?
-
 ?
validamine + 2,6-dichlorophenolindophenol 98.5% activity compared to D-glucose Sphingobacterium faecium ?
-
 ?
validamycin A + 2,6-dichlorophenolindophenol 28.1% activity compared to D-glucose Sphingobacterium faecium ?
-
 ?
validoxylamine A + 2,6-dichlorophenolindophenol 58.6% activity compared to D-glucose Sphingobacterium faecium ?
-
 ?
valienamine + 2,6-dichlorophenolindophenol 4.4% activity compared to D-glucose Sphingobacterium faecium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 63500, calculated from amino acid sequence Sphingobacterium faecium
? x * 62300, SDS-PAGE Sphingobacterium faecium

Synonyms

Synonyms Comment Organism
G3DH
-
 Sphingobacterium faecium
glucoside 3-dehydrogenase
-
 Sphingobacterium faecium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 the enzyme activity decreases significantly at 55 and 60°C, and 67.8% of enzyme activity is maintained when the enzyme is incubated at 50°C for 120 min Sphingobacterium faecium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Sphingobacterium faecium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6.6 the enzyme remains stable at pH 5.0-6.6 for 24 h Sphingobacterium faecium

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Sphingobacterium faecium