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Literature summary for 1.1.99.12 extracted from

  • Saito, Y.; Ishil, Y.; Hayashi, H.; Imao, Y.; Akashi, T.; Yoshikawa, K.; Noguchi, Y.; Soeda, S.; Yoshida, M.; Niwa, M.; Hosodo, J.; Shimomura.
    Cloning of genes coding for L-sorbose and L-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production of 2-keto-L-gulonate, a precursor of L-ascorbic acid, in a recombinant G. oxydans strain (1997), Appl. Environ. Microbiol., 63, 454-460.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
strain T-100, expression in Escherichia coli and Gluconobacter oxydans G624 Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, SDS-PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-sorbose + acceptor Gluconobacter oxydans
-
 L-sorbosone + reduced acceptor
-
 ?
L-sorbose + acceptor Gluconobacter oxydans T-100
-
 L-sorbosone + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
 isolated from japanese persimmon
-
Gluconobacter oxydans T-100
-
 isolated from japanese persimmon
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-sorbose + acceptor
-
 Gluconobacter oxydans L-sorbosone + reduced acceptor
-
 ?
L-sorbose + acceptor
-
 Gluconobacter oxydans T-100 L-sorbosone + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Gluconobacter oxydans