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Literature summary for 1.1.99.1 extracted from
- Functional characterization and sequence analysis of choline dehydrogenase from Escherichia coli (2010), Genet. Eng. Biotechnol. J., 2010, 0000.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene betA, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain M15/pREP4, subcloning in strain JM109 | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61848 | - |
x * 61848, sequence calculation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | A7LLT8 | isolated from a finfish, gene betA | - |
| Escherichia coli TCJAR023 | A7LLT8 | isolated from a finfish, gene betA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombnant enzyme from Escherichia coli strain M15 by ammonium sulfate fractionation and anion exchange chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + phenazine methosulfate | - |
Escherichia coli | betaine aldehyde + reduced phenazine methosulfate | - |
? |  |
| choline + phenazine methosulfate | - |
Escherichia coli TCJAR023 | betaine aldehyde + reduced phenazine methosulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61848, sequence calculation | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| phenazine methosulfate | - |
Escherichia coli | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | sequence calculation | - |
5.45 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | in Escherichia coli, the biosynthetic pathway for the production of glycine betaine from choline involves choline dehydrogenase (betA), betaine aldehyde dehydrogenase (betB), a putative regulator (betI) and a choline transporter (betT), the genes are clustered in the bet operon | Escherichia coli |
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