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Literature summary for 1.1.98.7 extracted from

  • Krüger, T.; Weiland, S.; Boschanski, M.; Sinha, P.K.; Falck, G.; Meller, K.M.; Dierks, T.; Sewald, N.
    Conversion of serine-type aldehyde tags by the radical SAM protein AtsB from Methanosarcina mazei (2019), Chembiochem, 20, 2074-2078 .
    View publication on PubMed
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Application

Application Comment Organism
molecular biology enzymatic modification of a DARPin model protein carrying the aldehyde tag sequence SAPAR enables the production of a fluorescent conjugate. Thus proving its potential for bioconjugation chemistry. This tag system could be useful in orthogonal combination with the standard cysteine-type tag system targeted by formylglycine-generating enzyme, which is regularly utilized for protein labeling Methanosarcina mazei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + a [sulfatase]-L-serine Methanosarcina mazei
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 a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
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 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + a [sulfatase]-L-serine
-
 Methanosarcina mazei a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
-
 ?
S-adenosyl-L-methionine + a [sulfatase]-L-serine the enzyme converts nonclassical aldehyde tags of the SX(A/P)XR-type. The enzyme is able to modify the artificial serine-containing motif STAGR Methanosarcina mazei a [sulfatase]-3-oxo-L-alanine + 5'-deoxyadenosine + L-methionine
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 ?

Synonyms

Synonyms Comment Organism
AtsB
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 Methanosarcina mazei