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Literature summary for 1.1.98.7 extracted from

  • Grove, T.L.; Lee, K.H.; St Clair, J.; Krebs, C.; Booker, S.J.
    In vitro characterization of AtsB, a radical SAM formylglycine-generating enzyme that contains three [4Fe-4S] clusters (2008), Biochemistry, 47, 7523-7538.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Klebsiella pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Klebsiella pneumoniae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
[4Fe-4S]2+ cluster three [4Fe-4S]2+ clusters per enzyme molecule Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae Q9X758
-
-
Klebsiella pneumoniae ATCC 700721D Q9X758
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity chromatography under an atmosphere of nitrogen Klebsiella pneumoniae

Storage Stability

Storage Stability Organism
-80°C, 50 mM potassium HEPES, pH 7.5, 500 mM KCl, 10 mM DTT, and 30% glycerol Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity Klebsiella pneumoniae acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-L-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine
-
?
acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity Klebsiella pneumoniae ATCC 700721D acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-L-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine
-
?
additional information the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a cysteine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases. No reaction with Tyr-Tyr-Thr-Ser-Pro-Met-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH Klebsiella pneumoniae ?
-
?
additional information the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a cysteine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases. No reaction with Tyr-Tyr-Thr-Ser-Pro-Met-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH Klebsiella pneumoniae ATCC 700721D ?
-
?
Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine + dithionite a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity Klebsiella pneumoniae Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-3-oxo-L-Ala-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
-
?
Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine + dithionite a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity Klebsiella pneumoniae ATCC 700721D Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-3-oxo-L-Ala-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
-
?

Synonyms

Synonyms Comment Organism
anaerobic sulfatase-maturating enzyme
-
Klebsiella pneumoniae
AtsB
-
Klebsiella pneumoniae

General Information

General Information Comment Organism
metabolism involved in the pathway of sulfatase oxidation Klebsiella pneumoniae