Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Klebsiella pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Klebsiella pneumoniae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]2+ cluster | three [4Fe-4S]2+ clusters per enzyme molecule | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | Q9X758 | - |
- |
Klebsiella pneumoniae ATCC 700721D | Q9X758 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity chromatography under an atmosphere of nitrogen | Klebsiella pneumoniae |
Storage Stability | Organism |
---|---|
-80°C, 50 mM potassium HEPES, pH 7.5, 500 mM KCl, 10 mM DTT, and 30% glycerol | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine | a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity | Klebsiella pneumoniae | acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-L-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine | - |
? | |
acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine | a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity | Klebsiella pneumoniae ATCC 700721D | acetyl-Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-L-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine | - |
? | |
additional information | the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a cysteine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases. No reaction with Tyr-Tyr-Thr-Ser-Pro-Met-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH | Klebsiella pneumoniae | ? | - |
? | |
additional information | the enzme also perfoms the reaction of the Cys-type anaerobic sulfatase-maturating enzyme, converting a cysteine residue of sulfatase into a 3-oxo-L-alanine residue. The post-translational modification of sulfatases, i.e. the creation of a 3-oxo-L-alanine (i.e. Calpha-formylglycine) residue from a cysteine or serine is vital for the proper function of sulfatases. No reaction with Tyr-Tyr-Thr-Ser-Pro-Met-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn-COOH | Klebsiella pneumoniae ATCC 700721D | ? | - |
? | |
Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine + dithionite | a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity | Klebsiella pneumoniae | Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-3-oxo-L-Ala-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O | - |
? | |
Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine + dithionite | a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite with higly reduced activity | Klebsiella pneumoniae ATCC 700721D | Tyr-Tyr-Thr-Ser-Pro-Met-Ser-Ala-Pro-Ala-Arg-3-oxo-L-Ala-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
anaerobic sulfatase-maturating enzyme | - |
Klebsiella pneumoniae |
AtsB | - |
Klebsiella pneumoniae |
General Information | Comment | Organism |
---|---|---|
metabolism | involved in the pathway of sulfatase oxidation | Klebsiella pneumoniae |