General Stability | Organism |
---|---|
unstable in 40 mM phosphate or Tris-HC1 buffer at 4°C and also in low-ionic-strength buffers in which precipitation occurrs and enzyme activity is lost irreversibly. The enzyme can be stabilized by addition of 200 mM sodium sulfate and 10 mM 2-propanol to the buffer | Methanofollis liminatans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 5 mM, 87% inhibition | Methanofollis liminatans | |
2,2'-dipyridyl | 2 mM, 18% inhibition | Methanofollis liminatans | |
4-hydroxymercuribenzoate | 0.1 mM, complete inhibition | Methanofollis liminatans | |
KCl | - |
Methanofollis liminatans | |
additional information | no inhibition be EDTA (2-5 mM), iodoacetate (2-5 mM), or 4-hydroxymercurobenzoate (0.01-0.1 mM) | Methanofollis liminatans | |
NaCl | - |
Methanofollis liminatans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
reduced coenzyme F420 | pH 5.8, 37°C | Methanofollis liminatans | |
0.018 | - |
oxidized coenzyme F420 | pH 5.8, 37°C | Methanofollis liminatans | |
0.5 | - |
propanone | pH 5.8, 37°C | Methanofollis liminatans | |
0.8 | - |
(S)-2-butanol | pH 5.8, 37°C | Methanofollis liminatans | |
1.2 | - |
2-butanol | pH 5.8, 37°C | Methanofollis liminatans | |
2.01 | - |
(R)-2-butanol | pH 5.8, 37°C | Methanofollis liminatans | |
2.2 | - |
2-propanol | pH 5.8, 37°C | Methanofollis liminatans | |
4.3 | - |
Cyclopentanone | pH 5.8, 37°C | Methanofollis liminatans | |
6.1 | - |
Cyclopentanol | pH 5.8, 37°C | Methanofollis liminatans | |
7.2 | - |
2-Pentanol | pH 5.8, 37°C | Methanofollis liminatans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme contains no zinc ions | Methanofollis liminatans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
4 * 39000, SDS-PAGE | Methanofollis liminatans |
150000 | - |
gel filtration | Methanofollis liminatans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanofollis liminatans | - |
- |
- |
Methanofollis liminatans DSM 4140 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanofollis liminatans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-butanol + oxidized coenzyme F420 | - |
Methanofollis liminatans | 2-butanone + reduced coenzyme F420 | - |
r | |
(R)-2-butanol + oxidized coenzyme F420 | - |
Methanofollis liminatans DSM 4140 | 2-butanone + reduced coenzyme F420 | - |
r | |
(S)-2-butanol + oxidized coenzyme F420 | - |
Methanofollis liminatans | 2-butanone + reduced coenzyme F420 | - |
r | |
(S)-2-butanol + oxidized coenzyme F420 | - |
Methanofollis liminatans DSM 4140 | 2-butanone + reduced coenzyme F420 | - |
r | |
2-butanone + reduced coenzyme F420 | - |
Methanofollis liminatans | 2-butanol + oxidized coenzyme F420 | - |
r | |
2-butanone + reduced coenzyme F420 | - |
Methanofollis liminatans DSM 4140 | 2-butanol + oxidized coenzyme F420 | - |
r | |
2-pentanol + oxidized coenzyme F420 | - |
Methanofollis liminatans | 2-pentanone + reduced coenzyme F420 | - |
r | |
2-pentanol + oxidized coenzyme F420 | - |
Methanofollis liminatans DSM 4140 | 2-pentanone + reduced coenzyme F420 | - |
r | |
2-pentanone + reduced coenzyme F420 | - |
Methanofollis liminatans | 2-pentanol + oxidized coenzyme F420 | - |
r | |
2-propanol + oxidized coenzyme F420 | - |
Methanofollis liminatans | 2-propanone + reduced coenzyme F420 | 2-propanone i.e. acetone | r | |
cyclopentanol + oxidized coenzyme F420 | - |
Methanofollis liminatans | cyclopentanone + reduced coenzyme F420 | - |
r | |
cyclopentanone + reduced coenzyme F420 | - |
Methanofollis liminatans | cyclopentanol + oxidized coenzyme F420 | - |
r | |
additional information | the enzyme catalysed the oxidation of various secondary and cyclic alcohols to the corresponding ketones and the reverse reaction. No primary alcohols are oxidized. 1,3-butandiol, glycerol, methanol, ethanol, 1,2-ethandiol, 1 -propanol, 1-amino-2-propanol, 1-butanol, 1-pentanol, 1-hexanol and benzylalcohol are not oxidized nor are the respective aldehydes or ketones reduced | Methanofollis liminatans | ? | - |
? | |
additional information | the enzyme catalysed the oxidation of various secondary and cyclic alcohols to the corresponding ketones and the reverse reaction. No primary alcohols are oxidized. 1,3-butandiol, glycerol, methanol, ethanol, 1,2-ethandiol, 1 -propanol, 1-amino-2-propanol, 1-butanol, 1-pentanol, 1-hexanol and benzylalcohol are not oxidized nor are the respective aldehydes or ketones reduced | Methanofollis liminatans DSM 4140 | ? | - |
? | |
propanone + reduced coenzyme F420 | 2-propanone i.e. acetone | Methanofollis liminatans | 2-propanol + oxidized coenzyme F420 | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 39000, SDS-PAGE | Methanofollis liminatans |
Synonyms | Comment | Organism |
---|---|---|
F420-dependent alcohol dehydrogenase | - |
Methanofollis liminatans |
secondary alcohol:F420 oxidoreductase | - |
Methanofollis liminatans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Methanofollis liminatans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
2-Pentanone | pH 5.8, 37°C | Methanofollis liminatans | |
2.1 | - |
2-butanone | pH 5.8, 37°C | Methanofollis liminatans | |
4.4 | - |
Cyclopentanone | pH 5.8, 37°C | Methanofollis liminatans | |
12.8 | - |
Cyclopentanol | pH 5.8, 37°C | Methanofollis liminatans | |
23.1 | - |
2-Pentanol | pH 5.8, 37°C | Methanofollis liminatans | |
38.5 | - |
(S)-2-butanol | pH 5.8, 37°C | Methanofollis liminatans | |
49 | - |
2-butanol | pH 5.8, 37°C | Methanofollis liminatans | |
51.3 | - |
(R)-2-butanol | pH 5.8, 37°C | Methanofollis liminatans | |
85 | - |
2-propanol | pH 5.8, 37°C | Methanofollis liminatans | |
85 | - |
oxidized coenzyme F420 | pH 5.8, 37°C | Methanofollis liminatans | |
161.5 | - |
reduced coenzyme F420 | pH 5.8, 37°C | Methanofollis liminatans | |
161.5 | - |
propanone | pH 5.8, 37°C | Methanofollis liminatans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Methanofollis liminatans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
Cyclopentanone | pH 5.8, 37°C | Methanofollis liminatans | |
2.1 | - |
Cyclopentanol | pH 5.8, 37°C | Methanofollis liminatans | |
3.2 | - |
2-Pentanol | pH 5.8, 37°C | Methanofollis liminatans | |
26 | - |
(R)-2-butanol | pH 5.8, 37°C | Methanofollis liminatans | |
38.6 | - |
2-propanol | pH 5.8, 37°C | Methanofollis liminatans | |
40.8 | - |
2-butanol | pH 5.8, 37°C | Methanofollis liminatans | |
48 | - |
(S)-2-butanol | pH 5.8, 37°C | Methanofollis liminatans | |
323 | - |
2-Pentanone | pH 5.8, 37°C | Methanofollis liminatans | |
4700 | - |
oxidized coenzyme F420 | pH 5.8, 37°C | Methanofollis liminatans | |
20200 | - |
reduced coenzyme F420 | pH 5.8, 37°C | Methanofollis liminatans |